ArticlePloS one2020
UltraPrep is a scalable, cost-effective, bead-based method for purifying cell-free DNA.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Fragmentation patterns of human telomeric chromatin in plasma cfDNA.Nature communications · 2026Article
- Dual ctDNA and CTCs analysis for minimal residual disease detection and relapse monitoring in early-stage breast cancer.NPJ breast cancer · 2026Article
- Cell-Free DNA-Based Methods to Map Chromatin.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Magnetic Nanoparticle-Based DNA Isolation from Refined Soybean Oil: A Cost-Effective Approach for GM Testing.Foods (Basel, Switzerland) · 2025Article
- Increased blood draws for ultrasensitive ctDNA and CTCs detection in early breast cancer patients.NPJ breast cancer · 2024Article
- Genetic Diagnosis of Retinoblastoma Using Aqueous Humour-Findings from an Extended Cohort.Cancers · 2024Article
- Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review.Biosensors · 2023Review
- Rapid and Efficient Extraction of Cell-Free DNA Using Homobifunctional Crosslinkers.Biomedicines · 2022Article
- Analytical Technologies for Liquid Biopsy of Subcellular Materials.Annual review of analytical chemistry (Palo Alto, Calif.) · 2021Article
- Technical and Methodological Aspects of Cell-Free Nucleic Acids Analyzes.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
UltraPrep is an open-source, two-step method for purification of cell-free DNA that entails extraction of total DNA followed by size-selective enrichment of the smaller fragments that are characteristic of DNA originating from fragmentation between nucleosome. The advantages of the two related protocols that are described are that they can easily accommodate a wide range of sample input volumes, they rely on simple, magnetic bead-based technology, the yields of cfDNA are directly comparable to the most popular methods for cfDNA purification, and they dramatically reduce the cost of cfDNA isolation relative to currently available commercial methods. We provide a framework for physical and molecular quality analysis of purified cfDNA and demonstrate that the cfDNA generated by UltraPrep meets or exceeds the quality metrics of the most commonly used procedure. In addition, our method removes high molecular weight genomic DNA (hmwgDNA) that can interfere with downstream assay results, thereby addressing one of the primary concerns for preanalytical collection of blood samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.