ArticlePloS one2020
Whole blood transcriptional responses of very preterm infants during late-onset sepsis.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
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Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 27 citations in OpenAlex.
- Using molecular methods to diagnose, classify, and treat neonatal sepsis: a scoping review.Frontiers in pediatrics · 2025Pooled it
- Transcript host-RNA signatures to discriminate bacterial and viral infections in febrile children.Pediatric research · 2022Pooled it
- Systems biology approaches to unravelling neonatal sepsis and potential for precision diagnostics and therapeutics.Frontiers in pharmacology · 2026Review
- Characterising commensal and pathogenic staphylococcal interactions with neonatal and adult blood.Scientific reports · 2025Article
- Dual RNA isolation from blood: an optimized protocol for host and bacterial RNA purification for dual RNA-sequencing analysis in whole blood sepsis samples.Microbial genomics · 2025Article
- Role of DNA methylation in regulating inflammatory cytokine expression in neonates with late-onset sepsis.Frontiers in immunology · 2025Article
- Putative mRNA Biomarkers for the Eradication of Infection in an Equine Experimental Model of Septic Arthritis.Veterinary sciences · 2024Article
- Identifying immune signatures of sepsis to increase diagnostic accuracy in very preterm babies.Nature communications · 2024Article
- Donor white blood cell differential is the single largest determinant of whole blood gene expression patterns.Genomics · 2023Article
- Effects of Sepsis on Immune Response, Microbiome and Oxidative Metabolism in Preterm Infants.Children (Basel, Switzerland) · 2023Review
- The Role of Coagulase-Negative Staphylococci Biofilms on Late-Onset Sepsis: Current Challenges and Emerging Diagnostics and Therapies.Antibiotics (Basel, Switzerland) · 2023Review
- The Role of the Interleukin-1 Family in Complications of Prematurity.International journal of molecular sciences · 2023Review
- Gene filtering strategies for machine learning guided biomarker discovery using neonatal sepsis RNA-seq data.Frontiers in genetics · 2023Article
- Ablation of long noncoding RNA MALAT1 activates antioxidant pathway and alleviates sepsis in mice.Redox biology · 2022Article
- Transcriptome profiles discriminate between Gram-positive and Gram-negative sepsis in preterm neonates.Pediatric research · 2022Observational
- Look Who's Talking: Host and Pathogen Drivers ofInternational journal of molecular sciences · 2022Review
- The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes as well as preterm birth and neonatal mortality in mice†.Biology of reproduction · 2021Article
- Stratified Management for Bacterial Infections in Late Preterm and Term Neonates: Current Strategies and Future Opportunities Toward Precision Medicine.Frontiers in pediatrics · 2021Review
- Zebrafish larvae as experimental model to expedite the search for new biomarkers and treatments for neonatal sepsis.Journal of clinical and translational science · 2021Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundHost immune responses during late-onset sepsis (LOS) in very preterm infants are poorly characterised due to a complex and dynamic pathophysiology and challenges in working with small available blood volumes. We present here an unbiased transcriptomic analysis of whole peripheral blood from very preterm infants at the time of LOS.
methodsRNA-Seq was performed on peripheral blood samples (6-29 days postnatal age) taken at the time of suspected LOS from very preterm infants <30 weeks gestational age. Infants were classified based on blood culture positivity and elevated C-reactive protein concentrations as having confirmed LOS (n = 5), possible LOS (n = 4) or no LOS (n = 9). Bioinformatics and statistical analyses performed included pathway over-representation and protein-protein interaction network analyses. Plasma cytokine immunoassays were performed to validate differentially expressed cytokine pathways.
resultsThe blood leukocyte transcriptional responses of infants with confirmed LOS differed significantly from infants without LOS (1,317 differentially expressed genes). However, infants with possible LOS could not be distinguished from infants with no LOS or confirmed LOS. Transcriptional alterations associated with LOS included genes involved in pathogen recognition (mainly TLR pathways), cytokine signalling (both pro-inflammatory and inhibitory responses), immune and haematological regulation (including cell death pathways), and metabolism (altered cholesterol biosynthesis). At the transcriptional-level cytokine responses during LOS were characterised by over-representation of IFN-α/β, IFN-γ, IL-1 and IL-6 signalling pathways and up-regulation of genes for inflammatory responses. Infants with confirmed LOS had significantly higher levels of IL-1α and IL-6 in their plasma.
conclusionsBlood responses in very preterm infants with LOS are characterised by altered host immune responses that appear to reflect unbalanced immuno-metabolic homeostasis.
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