ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2021
Serum deprivation limits loss and promotes recovery of tenogenic phenotype in tendon cell culture systems.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Beyond Tissue Origin: Rethinking Experimental Model Selection in Rotator Cuff Research.Medical sciences (Basel, Switzerland) · 2026Article
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- Fetal bovine serum: how to leave it behind in the pursuit of more reliable science.Frontiers in toxicology · 2025Review
- GelMA hydrogel dual photo-crosslinking to dynamically modulate ECM stiffness.Frontiers in bioengineering and biotechnology · 2024Article
- Towards standardization of human adipose-derived stromal cells secretomes.Stem cell reviews and reports · 2023Review
- Mesenchymal Stem Cell Conditioned Medium Modulates Inflammation in Tenocytes: Complete Conditioned Medium Has Superior Therapeutic Efficacy than Its Extracellular Vesicle Fraction.International journal of molecular sciences · 2023Article
- Long-term follow-up and exploration of the mechanism of stromal vascular fraction gel in chronic wounds.Stem cell research & therapy · 2023Article
- The Stiffness-Sensitive Transcriptome of Human Tendon Stromal Cells.Advanced healthcare materials · 2023Article
- Dynamic Load Model Systems of Tendon Inflammation and Mechanobiology.Frontiers in bioengineering and biotechnology · 2022Review
- A combined physicochemical approach towards human tenocyte phenotype maintenance.Materials today. Bio · 2021Article
- Serum deprivation limits loss and promotes recovery of tenogenic phenotype in tendon cell culture systems.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Impact of isolation method on cellular activation and presence of specific tendon cell subpopulations during in vitro culture.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Inhibition of ERK 1/2 kinases prevents tendon matrix breakdown.Scientific reports · 2021Article
- Identifying potential patient-specific predictors for anterior cruciate ligament reconstruction outcome - a diagnostic in vitro tissue remodeling platform.Journal of experimental orthopaedics · 2020Article
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Authors and funding
5 authors.
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Abstract
Current knowledge gaps on tendon tissue healing can partly be ascribed to the limited availability of physiologically relevant culture models. An unnatural extracellular matrix, high serum levels and random cell morphology in vitro mimic strong vascularization and lost cell elongation in pathology, and discord with a healthy, in vivo cell microenvironment. The thereby induced phenotypic drift in tendon-derived cells (TDCs) compromises the validity of the research model. Therefore, this research quantified the extracellular matrix (ECM)-, serum-, and cell morphology-guided phenotypic changes in tendon cells of whole tendon fascicle explants with intact ECM and TDCs cultured in a controlled microenvironmental niche. Explanted murine tail tendon fascicles were cultured in serum-rich or serum-free medium and phenotype was assessed using transcriptome analysis. Next, phenotypic marker gene expression was measured in in vitro expanded murine tail TDCs upon culture in serum-rich or serum-free medium on aligned or random collagen I patterns. Freshly isolated fascicles or TDCs served as native controls. In both systems, the majority of tendon-specific genes were similarly attenuated in serum-rich culture. Strikingly, 1-week serum-deprived culture-independent of cell morphology-converged TDC gene expression toward native levels. This study reveals a dynamic serum-responsive tendon cell phenotype. Extracting fascicles or TDCs from their native environment causes large changes in cellular phenotype, which can be limited and even reversed by serum deprivation. We conclude that serum-derived factors override matrix-integrity and cell morphology cues and that serum-deprivation stimulates a more physiological microenvironment for in vitro studies.
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