Evidence map›Paper›PMID 32478788›Full record

ArticleGenetics and molecular biology2020

Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.).

Ming-Hua Deng, Kai Zhao, Jun-Heng Lv, Jin-Long Huo, Zhu-Qing Zhang, Hai-Shan Zhu, Xue-Xiao Zou, Jin-Fen Wen

Open access · goldAbstract read
In one paragraph

Article in Genetics and molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Gene Expression Analysis by Quantitative Real-Time PCR for Floral Tissues.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Ming-Hua DengCollege of Horticulture, Yunnan Agricultural University, Kunming, P. R. China.ORCID http://orcid.org/0000-0001-8293-9035
Kai ZhaoCollege of Horticulture, Yunnan Agricultural University, Kunming, P. R. China.
Jun-Heng LvHunan Academy of Agricultural Science, Changsha, P. R. China.
Jin-Long HuoFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, P. R. China.
Zhu-Qing ZhangHunan Academy of Agricultural Science, Changsha, P. R. China.
Hai-Shan ZhuCollege of Horticulture, Yunnan Agricultural University, Kunming, P. R. China.
Xue-Xiao ZouHunan Academy of Agricultural Science, Changsha, P. R. China.
Jin-Fen WenCollege of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA.
Yunnan Agricultural University · CNHunan Academy of Agricultural Sciences · CNKunming University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different developmental stages of pepper flower [sporogenous cell division, stage (B1); pollen mother cell meiosis, stage (B2); and open flower (B3)]. In the cDNA libraries for B1, B2, and B3: 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34,445 unigene sequences and 128 pathways were annotated by KEGG pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified, and 8,955, 12,182, and 23,667 DEGs were identified in the B2 vs B1, B3 vs B1, and B3 vs. B2 comparisons. DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. According to the RNA-seq data, all 13 selected DEGs showed similar expression patterns after q-PCR analysis. Sucrose-phosphatase, galactinol-sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The new results provide valuable genetic information about flower development in pepper.

Identifiers

PMID32478788
PMCPMC7263202
OpenAlexW3032148857

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.