ArticlePlant methods2020
Ice accommodation in plant tissues pinpointed by cryo-microscopy in reflected-polarised-light.
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Improved laser capture microdissection combined with transcriptome sequencing reveals gene expression reprogramming during rice panicle branch meristems differentiation.BMC plant biology · 2026Article
- Spatial and Temporal Freezing Dynamics of Leaves Revealed by Time-Lapse Imaging.Plant, cell & environment · 2025Article
- Frozen mountain pine needles: The endodermis discriminates between the ice-containing central tissue and the ice-free fully functional mesophyll.Physiologia plantarum · 2023Article
- Freeze dehydration vs. supercooling of mesophyll cells: Impact of cell wall, cellular and tissue traits on the extent of water displacement.Physiologia plantarum · 2022Article
- Repeated colonization of alpine habitats by Arabidopsis arenosa viewed through freezing resistance and ice management strategies.Plant biology (Stuttgart, Germany) · 2022Article
- Freezing induces an increase in leaf spectral transmittance of forest understorey and alpine forbs.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Article
- Winter survival of the unicellular green alga Micrasterias denticulata: insights from field monitoring and simulation experiments.Protoplasma · 2021Article
- Protective Role of Ice Barriers: How Reproductive Organs of Early Flowering and Mountain Plants Escape Frost Injuries.Plants (Basel, Switzerland) · 2021Article
- Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants.International journal of molecular sciences · 2020Article
- Winter Nights during Summer Time: Stress Physiological Response to Ice and the Facilitation of Freezing Cytorrhysis by Elastic Cell Wall Components in the Leaves of a Nival Species.International journal of molecular sciences · 2020Article
- Ice Nucleation and Freezing Consequences in Perennial Plants.Physiologia plantarumReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundFreezing resistant plant organs are capable to manage ice formation, ice propagation, and ice accommodation down to variable temperature limits without damage. Insights in ice management strategies are essential for the fundamental understanding of plant freezing and frost survival. However, knowledge about ice management is scarce. Ice crystal localisation inside plant tissues is challenging and is mainly based on optical appearance of ice in terms of colour and shape, investigated by microscopic methods. Notwithstanding, there are major uncertainties regarding the reliability and accuracy of ice identification and localisation. Surface light reflections, which can originate from water or resin, even at non-freezing temperatures, can have a similar appearance as ice. We applied the principle of birefringence, which is a property of ice but not of liquid water, in reflected-light microscopy to localise ice crystals in frozen plant tissues in an unambiguous manner.
resultsIn reflected-light microscopy, water was clearly visible, while ice was more difficult to identify. With the presented polarised cryo-microscopic system, water, including surface light reflections, became invisible, whereas ice crystals showed a bright and shiny appearance. Based on this, we were able to detect loci where ice crystals are accommodated in frozen and viable plant tissues. In
conclusionCryo-microscopy in reflected-polarised-light allowed a robust identification of ice crystals in frozen plant tissue. It distinguishes itself, compared with other methods, by its ease of ice identification, time and cost efficiency and the possibility for high throughput. Profound knowledge about ice management strategies, within the whole range of freezing resistance capacities in the plant kingdom, might be the link to applied science for creating arrangements to avoid future frost damage to crops.
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