Evidence map›Paper›PMID 32477058›Full record

ArticleFrontiers in neuroscience2020

Deep Brain Stimulation of the Medial Septal Nucleus Induces Expression of a Virally Delivered Reporter Gene in Dentate Gyrus.

Anton Fomenko, Darrin J Lee, Chris McKinnon, Eun Jung Lee, Mitchell L de Snoo, Elise Gondard, Clemens Neudorfer, Clement Hamani, Andres M Lozano, Lorraine V Kalia and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in neuroscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Anton FomenkoKrembil Research Institute, University Health Network, Toronto, ON, Canada.
Darrin J LeeDepartment of Neurological Surgery and USC Neurorestoration Center, Keck School of Medicine of USC, Los Angeles, CA, United States.
Chris McKinnonKrembil Research Institute, University Health Network, Toronto, ON, Canada.
Eun Jung LeeKrembil Research Institute, University Health Network, Toronto, ON, Canada.
Mitchell L de SnooKrembil Research Institute, University Health Network, Toronto, ON, Canada.
Elise GondardKrembil Research Institute, University Health Network, Toronto, ON, Canada.
Clemens NeudorferKrembil Research Institute, University Health Network, Toronto, ON, Canada.
Clement HamaniDivision of Neurosurgery, Sunnybrook Health Sciences Centre, University of Toronto, Toronto, ON, Canada.
Andres M LozanoKrembil Research Institute, University Health Network, Toronto, ON, Canada.
Lorraine V KaliaKrembil Research Institute, University Health Network, Toronto, ON, Canada.
Suneil K KaliaKrembil Research Institute, University Health Network, Toronto, ON, Canada.
University Health Network · CAKrembil Research InstituteNeurological Surgery · USSunnybrook Health Science Centre · CAToronto Western Hospital · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMechanisms of deep brain stimulation (DBS) remain controversial, and spatiotemporal control of brain-wide circuits remains elusive. Adeno-associated viral (AAV) vectors have emerged as vehicles for spatiotemporal expression of exogenous transgenes in several tissues, including specific nuclei in the brain. Coupling DBS with viral vectors to modulate exogenous transgene expression remains unexplored.

objectiveThis study examines whether DBS of the medial septal nucleus (MSN) can regulate gene expression of AAV-transduced neurons in a brain region anatomically remote from the stimulation target: the hippocampal dentate gyrus.

methodsRats underwent unilateral hippocampal injection of an AAV vector with c-Fos promoter-driven expression of TdTomato (TdT), followed by MSN electrode implantation. Rodents received no stimulation, 7.7 Hz (theta), or 130 Hz (gamma) DBS for 1 h one week after surgery. In a repeat stimulation experiment, rodents received either no stimulation, or two 1 h MSN DBS over 2 weeks.

resultsNo significant differences in hippocampal TdT expression between controls and acute MSN DBS were found. With repeat DBS we found c-Fos protein expression was induced and we could detect increased TdT with either gamma or theta stimulation.

conclusionWe demonstrate that viral vector-mediated gene expression can be regulated spatially and temporally using DBS. Control of gene expression by DBS warrants further investigation into stimulation-responsive promoters for clinical applications.

Indexed as

deep brain stimulationgene expressionhippocampusmedial septal nucleusneuromodulationviral vector

Identifiers

PMID32477058
PMCPMC7235415
OpenAlexW3021666300

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.