Evidence map›Paper›PMID 32476275›Full record

ArticleJournal of cellular and molecular medicine2020

Exosomal microRNA-26b-5p down-regulates ATF2 to enhance radiosensitivity of lung adenocarcinoma cells.

Fushi Han, Dongdong Huang, Xinghong Huang, Wei Wang, Shusong Yang, Shuzhen Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 1 pooled it
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Fushi HanDepartment of Nuclear Medicine, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
Dongdong HuangDepartment of Emergency Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Xinghong HuangDepartment of Radiology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
Wei WangDepartment of Internal Medicine, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
Shusong YangDepartment of Radiotherapy, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
Shuzhen ChenDepartment of Nuclear Medicine, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.ORCID 0000-0003-3041-5541
Tongji University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lung adenocarcinoma (LUAD), as the most common subtype of non-small cell lung cancer, is responsible for more than 500 000 deaths worldwide annually. In this study, we identify a novel microRNA-26b-5p (miR-26b-5p) and elucidated its function on LUAD. The survival rate of parent LUAD cells and radiation-resistant LUAD cells were determined using clonogenic survival assay. We overexpressed or inhibited miR-26b-5p in LUAD, and the correlation between activating transcription factor 2 (ATF2) and miR-26b-5p was determined using integrated bioinformatics analysis and dual-luciferase reporter gene assay. Exosomes derived from A549 cell lines were then detected using Western blot assay, followed by co-transfection with radiation-resistant A549R cells. LUAD tissues and serum were collected, followed by miR-26b-5p relative expression quantification using RT-qPCR. miR-26b-5p was identified as the most differentially expressed miRNA and was down-regulated in LUAD. Radiation-resistant cells were more resistant to X-radiation compared with parent cells. miR-26b-5p overexpression and X-irradiation led to enhanced radiosensitivity of LUAD cells. ATF2 was negatively targeted by miR-26b-5p. Exosomal miR-26b-5p derived from A549 cells could be transported to irradiation-resistant LUAD cells and inhibit ATF2 expression to promote DNA damage, apoptosis and radiosensitivity of LUAD cells, which was verified using serum-based miR-26b-5p. Our results show a regulatory network of miR-26b-5p on radiosensitivity of LUAD cells, which may serve as a non-invasive biomarker for LUAD.

Indexed as

Gene Expression Regulation, NeoplasticA549 CellsActivating Transcription Factor 2Adenocarcinoma of LungAnimalsApoptosisBiomarkersCell Line, TumorCell SurvivalDisease Models, AnimalExosome Multienzyme Ribonuclease ComplexHumansMiceMicroRNAsPrognosisRadiation ToleranceActivating Transcription Factor 2ATF2 protein, humanBiomarkersExosome Multienzyme Ribonuclease ComplexMicroRNAsMIRN26 microRNA, humanactivating transcription factor 2exosomeslung adenocarcinomalung adenocarcinoma cellsmicroRNA-26b-5pradiosensitivity

Identifiers

PMID32476275
PMCPMC7348161
OpenAlexW3097114559

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.