ArticlePoultry science2020
Effects of lysine and methionine in a low crude protein diet on the growth performance and gene expression of immunity genes in broilers.
Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Gut microbiota in poultry: Roles in nutrition, metabolism and production performance.Poultry science · 2026Review
- Effects of Fibrafid Supplementation in Standard and Nutrient-Reduced Diets on Growth Performance, Production Efficiency, Carcass Traits, Meat Quality, and Intestinal Morphology in Broilers.Veterinary sciences · 2026Article
- Effects of Dietary Standardized Ileal Digestible Lysine and Amylose/Amylopectin Ratio on Intestinal Morphology, Barrier-Related Gene Expression, and Cecal Microbiota in Broilers Fed Low-Protein Diets.Animals : an open access journal from MDPI · 2026Article
- Effects of Amino Acid Supplementation in Low-Protein Diets on Productive Performance, Digestive Function, and Intestinal Health of Laying Hens.Animals : an open access journal from MDPI · 2026Article
- Dietary Amylose/Amylopectin Ratio Modulated Growth Performance, Intestinal Health, and Lipid Metabolism in Goslings Fed Low-Protein Diets.Animals : an open access journal from MDPI · 2026Article
- Comparative gut microbiota, growth performances, and cytokine indices in broiler chickens with or without litter.Journal of animal science and technology · 2025Article
- Taste Preferences in Broilers: Behavioral Evaluation for Varying Concentrations of Four Essential Amino Acids.Animals : an open access journal from MDPI · 2025Article
- Slowly digestible starch impairs growth performance of broiler chickens offered low-protein diet supplemental higher amino acid densities by inhibiting the utilization of intestinal amino acid.Journal of animal science and biotechnology · 2025Article
- An optimised promoter and signal peptide improves methionine production of a genetically engineeredFrontiers in microbiology · 2025Article
- Determination and Prediction of Amino Acid Digestibility in Rapeseed Cake for Growing-Finishing Pigs.Animals : an open access journal from MDPI · 2024Article
- Dietary Crude Protein and Lysine Levels Affect Meat Quality and Myofiber Characteristic of Slow-Growing Chicken.Animals : an open access journal from MDPI · 2024Article
- Optimization of Fermented Maize Stover for the Fattening Phase of Geese: Effect on Production Performance and Gut Microflora.Animals : an open access journal from MDPI · 2024Article
- Dietary supplementation withFrontiers in veterinary science · 2024Article
- Feeding Behavior, Growth Performance and Meat Quality Profile in Broiler Chickens Fed Multiple Levels of Xylooligosaccharides.Animals : an open access journal from MDPI · 2023Article
- Different amino acid supplementation patterns in low-protein diets on growth performance and nitrogen metabolism of goslings from 1 to 28 days of age.Poultry science · 2023Article
- Impacts of Dietary Lysine and Crude Protein on Performance, Hepatic and Renal Functions, Biochemical Parameters, and Histomorphology of Small Intestine, Liver, and Kidney in Broiler Chickens.Veterinary sciences · 2023Article
- Chicken adaptive response to nutrient density: immune function change revealed by transcriptomic analysis of spleen.Frontiers in immunology · 2023Article
- Protease or Clostridium butyricum addition to a low-protein diet improves broiler growth performance.Applied microbiology and biotechnology · 2022Article
- Dietary Soy Saponin Improves Antioxidant and Immune Function of Layer Hens.The journal of poultry science · 2022Article
- Lysine Depletion during Different Feeding Phases: Effects on Growth Performances and Meat Quality of Broiler Chickens.Animals : an open access journal from MDPI · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Globally, the poultry industry is 1 of the most advanced livestock industries. Feed contributes to the biggest proportion (65-70%) of the production cost. Most feed ingredients in Malaysia are imported, which contributes to the high food bill annually, and alternative feed formulation may help decrease the cost of poultry feed. Feed formulation are improved to efficiently meet the dietary requirements of the broilers and 1 of the ways is by reducing the level of crude protein in the diet while supplementing essential amino acids. In this study, the effects of methionine and lysine, which are the 2 most limiting amino acids in the chicken diet, were supplemented in a low crude protein diet, and its effects on the growth and expression of immunity genes such as MUC2, SLC, GAL6, and LEAP-2 were studied. A total of 300 Cobb500 broilers were tested with 10 different dietary treatments. Experimental treatment diets consist of high, standard, and low levels of methionine and lysine in the diet with reduced crude protein. The control group consists of diet with standard levels of lysine, methionine, and crude protein as recommended for Cobb500 broilers. Ribonucleic acid was extracted from the jejunum, spleen, and liver for gene expression analysis which was performed with real-time polymerase chain reaction using SYBR Green chemistry. Results of the growth performance at 6 wk showed improved feed conversion ratio when lysine was increased by 0.2% in a low crude protein diet at 1.96 ± 0.11. Gene expression of MUC2 gene in the jejunum showed a significant increase across all experimental diets with the treatment with higher lysine in low crude protein diet with the highest increase of 3.8 times as compared with the control diet. The other genes expressed in the spleen and liver were mostly downregulated. It was concluded that supplementation of high lysine with standard methionine in a low crude protein diet performed better in terms of lowest feed conversion ratio and high upregulation of MUC2 gene.
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