Evidence map›Paper›PMID 32474893›Full record

ArticleInfectious diseases and therapy2020

Challenges to Differentiate Hepatitis C Genotype 1 and 6: Results from A Field-Study in Cambodia.

Anja De Weggheleire, Irith De Baetselier, Sokkab An, Sylvie Goletti, Vanessa Suin, Sopheak Thai, Sven Francque, Tania Crucitti, Lutgarde Lynen, Steven Van Gucht and 1 more

Registry-linked trialOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infectious diseases and therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02361541 (Determination of Hepatitis C Prevalence, Genetic Diversity and Treatment Eligibility in an HIV Patient Cohort in Phnom Penh, Cambodia), which is not on this map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02361541 completednot on this map

Determination of Hepatitis C Prevalence, Genetic Diversity and Treatment Eligibility in an HIV Patient Cohort in Phnom Penh, Cambodia

TypeobservationalSponsorInstitute of Tropical Medicine, BelgiumRan2014 to 2016Enrolled3,045ConditionsHepatitis C, HIVArmsHCV screening
3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Anja De WeggheleireClinical Sciences Department, Institute of Tropical Medicine Antwerp, Antwerp, Belgium. adeweggheleire@itg.be.ORCID http://orcid.org/0000-0001-9377-4766
Irith De BaetselierClinical Sciences Department, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.
Sokkab AnInfectious Diseases Department, Sihanouk Hospital Center of Hope, Phnom Penh, Cambodia.
Sylvie GolettiClinical Microbiology, Cliniques Universitaires Saint Luc, Brussels, Belgium.
Vanessa SuinViral Diseases Department, Sciensano, Brussels, Belgium.
Sopheak ThaiInfectious Diseases Department, Sihanouk Hospital Center of Hope, Phnom Penh, Cambodia.
Sven FrancqueGastroenterology Hepatology, Antwerp University Hospital, Antwerp, Belgium.
Tania CrucittiClinical Sciences Department, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.
Lutgarde LynenClinical Sciences Department, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.
Steven Van GuchtViral Diseases Department, Sciensano, Brussels, Belgium.
Benoît Mukadi KabambaClinical Microbiology, Cliniques Universitaires Saint Luc, Brussels, Belgium.
Instituut voor Tropische Geneeskunde · BECliniques Universitaires Saint-Luc · BESciensano (Belgium) · BESihanouk Hospital Center of HOPE · KHUniversity of Antwerp · BE

Funding

Departement Economie, Wetenschap en Innovatie SOFI-Hepatitis CDirectorate-General for International Cooperation and Development Framework Agreement
6 · The paper itself

Abstract

introductionWe aim to report on results and challenges of different methods used for hepatitis C (HCV) genotyping in a Cambodian HCV/HIV coinfection project.

methodsSamples of 106 patients were available. HCV genotyping was initially (63 samples) done by the LightPower Taqman real-time PCR method (Viet A Corp.) and quality controlled using the Versant 2.0 line probe assay (Siemens Healthcare). Next, following interim quality control results, all 106 samples were (re)genotyped with Versant 2.0, complemented with 5'UTR/core sequencing for uninterpretable/incomplete Versant results.

resultsUsing Versant, 103 (97.2%) of the 106 HCV-coinfected patients had an interpretable genotype result: 1b (50.5%), 6 non-a/non-b (30.1%), 1a (6.8%), 6a or b (4.9%), 2 (3.9%), 1 (2.9%) and 3 (1.0%). For 16 samples that were interpreted as genotype 1 or 1b per Versant's current instructions, it could not be excluded that it concerned a genotype 6 infection as the core region line patterns on the Versant test strip were unavailable, inconclusive or atypical. Upon sequencing, seven of these were genotyped as 1b and nine as genotype 6. Combining Versant and sequencing results, a definitive genotype was assigned in 104 patients: 1b (44.2%), 6 non-a/non-b (39.4%), 1a (6.7%), 6a or b (4.8%), 2 (3.8%) and 3 (1.0%). Genotyping by LightPower and Versant was discordant for 23 (of 63) samples. The LightPower assay misclassified all genotype 6 non-a/non-b samples as genotype 1, which indicates that this assay is only using 5'UTR information.

conclusionsHCV genotype 1b and genotype 6 non-a/non-b were most common. With Versant 2.0 (using 5'UTR and core information), genotype classification (1 or 6) remained inconclusive in 15% of samples. The locally available method (LightPower assay) failed to identify genotype 6 non-a/non-b, which highlights that methods using 5'UTR information only should not be used in Cambodia. Regional/national guidelines should be explicit about this.

trial registrationThis study was performed as part of a larger cross-sectional study on the burden of hepatitis C coinfection in HIV patients in Cambodia (Clinical.trials.gov: HCV-Epi NCT02361541).

Indexed as

Diagnostic methodsGenotypingHepatitis CMisclassificationSoutheast Asia

Identifiers

PMID32474893
PMCPMC7452997
OpenAlexW3031577253

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.