ArticleApplied microbiology and biotechnology2020
ARTP mutation and adaptive laboratory evolution improve probiotic performance of Bacillus coagulans.
Article in Applied microbiology and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Screening and ARTP mutagenesis-based breeding of high-cellulase-producing strains.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Breeding of High-Polysaccharide-ProducingJournal of fungi (Basel, Switzerland) · 2025Article
- Improving the bacillomycin L production in Bacillus amyloliquefaciens by atmospheric and room-temperature plasma combined with Box-Behnken design.Microbial cell factories · 2025Article
- ARTP mutagenesis for genome-wide identification of genes important for biofilm regulation in spoilage bacteriumApplied and environmental microbiology · 2025Article
- Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing.Bioresources and bioprocessing · 2025Review
- Evolutionary adaptation of probiotics in the gut: selection pressures, optimization strategies, and regulatory challenges.NPJ biofilms and microbiomes · 2025Review
- Engineering Useful Microbial Species for Pharmaceutical Applications.Microorganisms · 2025Review
- Bacillus coagulans alleviates hepatic injury caused by Klebsiella pneumoniae in rabbits.PloS one · 2025Article
- Combinatorial mutagenesis and fermentation optimization biotechnologies synergistically enhance monacolin K content in functional red yeast rice.Frontiers in microbiology · 2025Article
- Research Progress in Mutagenesis Techniques forMini reviews in medicinal chemistry · 2025Review
- Analysis of oil accumulation mechanisms in plasma induced mutant Scenedesmus strains compared to original Scenedesmus strains.Scientific reports · 2024Article
- Furfural tolerance of mutant Saccharomyces cerevisiae selected via ionizing radiation combined with adaptive laboratory evolution.Biotechnology for biofuels and bioproducts · 2024Article
- ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants.Archives of microbiology · 2024Article
- Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution.Applied microbiology and biotechnology · 2024Article
- Mutagenesis selection and large-scale cultivation of non-greenFrontiers in nutrition · 2024Article
- Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding.Current issues in molecular biology · 2023Review
- Cold plasma pretreatment reinforces the lignocellulose-derived aldehyde inhibitors tolerance and bioethanol fermentability for Zymomonas mobilis.Biotechnology for biofuels and bioproducts · 2023Article
- Enhancing wuyiencin productivity of Streptomyces albulus (CK15) by mutagenesis breeding with atmospheric and room temperature plasma.World journal of microbiology & biotechnology · 2023Article
- Recent progress in adaptive laboratory evolution of industrial microorganisms.Journal of industrial microbiology & biotechnology · 2023Article
- Rapid Screening of High-Yield Gellan Gum Mutants ofFoods (Basel, Switzerland) · 2022Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Bacillus coagulans is a thermophilic, facultative anaerobic, spore-forming Gram-positive bacterium, which is used as a probiotic in animal feed and human dietary supplements. In the present study, a bile-resistant thermophilic B. coagulans WT-03 strain was isolated and genetically identified. Atmospheric pressure room temperature plasma (ARTP)-induced mutation combined with adaptive laboratory evolution (ALE) was used to improve the probiotic performance of B. coagulans WT-03. After 15 s of ARTP mutation and 40 days of ALE culture, a mutant artp-aleBC15 was obtained and showed the improved tolerance to pH 2.5 and 0.3% bile salt with a survival rate of 22.4%. Further studies showed that the artp-aleBC15 mutant exhibited a relatively stable morphology, lower permeability, and higher hydrophobicity of cell membrane compared with the parent strain of B. coagulans. Additionally, artp-aleBC15 could maintain homeostasis with an intracellular pH of over 4.5 and had the altered contents of saturated fatty acids/unsaturated fatty acids in the cell membrane at pH 2.5. Our study proved that ARTP mutation combined with ALE is an efficient mutagenesis strategy to improve the probiotic performance of B. coagulans for potential industrial use.Key Points• A B. coagulans strain that can grow at 80 °C and 0.3% bile salt was screened.• ARTP combined with ALE effectively mutated B. coagulans WT-03.• B. coagulans artp-aleBC15 mutant showed an improved probiotic performance.• The mutant exhibited the lower permeability and altered fatty acid contents in the cell membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.