ArticleMetabolic engineering2020
Reverse engineering of fatty acid-tolerant Escherichia coli identifies design strategies for robust microbial cell factories.
Article in Metabolic engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- An Engineered Variant ofACS synthetic biology · 2026Article
- Efficient production of salicylic acid through CmeR-PBiotechnology for biofuels and bioproducts · 2025Article
- Resistance mechanism of Escherichia coli strains with different ampicillin resistance levels.Applied microbiology and biotechnology · 2024Article
- Genome reduction improves octanoic acid production in scale down bioreactors.Microbial biotechnology · 2024Article
- The hallmarks of a tradeoff in transcriptomes that balances stress and growth functions.mSystems · 2024Article
- Strategies to increase the robustness of microbial cell factories.Advanced biotechnology · 2024Review
- Deep mutational scanning reveals the molecular determinants of RNA polymerase-mediated adaptation and tradeoffs.Nature communications · 2023Article
- Beyond rational-biosensor-guided isolation of 100 independently evolved bacterial strain variants and comparative analysis of their genomes.BMC biology · 2023Article
- The hallmarks of a tradeoff in transcriptomes that balances stress and growth functions.Research square · 2023Article
- Recent progress in adaptive laboratory evolution of industrial microorganisms.Journal of industrial microbiology & biotechnology · 2023Article
- Revealing novel synergistic defense and acid tolerant performance of Escherichia coli in response to organic acid stimulation.Applied microbiology and biotechnology · 2022Article
- Gene Coexpression Connectivity Predicts Gene Targets Underlying High Ionic-Liquid Tolerance in Yarrowia lipolytica.mSystems · 2022Article
- Strategies to increase tolerance and robustness of industrial microorganisms.Synthetic and systems biotechnology · 2022Article
- Article
- Microbial production of advanced biofuels.Nature reviews. Microbiology · 2021Review
- The challenges and prospects of Escherichia coli as an organic acid production host under acid stress.Applied microbiology and biotechnology · 2021Review
- Mutations in adaptively evolved Escherichia coli LGE2 facilitated the cost-effective upgrading of undetoxified bio-oil to bioethanol fuel.Bioresources and bioprocessing · 2021Article
- Possible Roles for Basal Levels of (p)ppGpp: Growth Efficiency Vs. Surviving Stress.Frontiers in microbiology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
Adaptive laboratory evolution is often used to improve the performance of microbial cell factories. Reverse engineering of evolved strains enables learning and subsequent incorporation of novel design strategies via the design-build-test-learn cycle. Here, we reverse engineer a strain of Escherichia coli previously evolved for increased tolerance of octanoic acid (C8), an attractive biorenewable chemical, resulting in increased C8 production, increased butanol tolerance, and altered membrane properties. Here, evolution was determined to have occurred first through the restoration of WaaG activity, involved in the production of lipopolysaccharides, then an amino acid change in RpoC, a subunit of RNA polymerase, and finally mutation of the BasS-BasR two component system. All three mutations were required in order to reproduce the increased growth rate in the presence of 20 mM C8 and increased cell surface hydrophobicity; the WaaG and RpoC mutations both contributed to increased C8 titers, with the RpoC mutation appearing to be the major driver of this effect. Each of these mutations contributed to changes in the cell membrane. Increased membrane integrity and rigidity and decreased abundance of extracellular polymeric substances can be attributed to the restoration of WaaG. The increase in average lipid tail length can be attributed to the RpoC
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.