ArticleParasites & vectors2020
Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 5 citations in OpenAlex.
- Natural Infection by Toscana Virus and Other Phleboviruses in Wild Sand Flies From Spain: Prevalence, Genetic Characterization and Tripartite Interactions With Leishmania infantum and Wolbachia.Zoonoses and public health · 2026Article
- Retrospective entomological and eco-epidemiological survey of a leishmaniasis outbreak in southern Spain.One health (Amsterdam, Netherlands) · 2026Article
- Development of a new quantitative RT-PCR to detect lymphocytic choriomeningitis virus.Frontiers in veterinary science · 2025Article
- Development of a Real-Time PCR Assay for the Detection of the Phlebovirus Fermo Virus.Viruses · 2023Article
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
Abstract
backgroundGranada virus belongs to the genus Phlebovirus within the Naples serocomplex and was detected for the first time in sand flies from Spain in 2003. Seroprevalence studies have revealed that Granada virus may infect humans with most cases being asymptomatic. Moreover, recent studies in vector samples revealed that the related Massilia and Arrabida phleboviruses could be also circulating in Spain. The objective of this study was to develop and assess a new sensitive real-time RT-PCR assay for Granada virus diagnosis able to detect the related phleboviruses Massilia and Arrabida.
methodsTwo specific primers and one unique probe to detect Granada, Massilia and Arrabida viruses, without differentiating between them, were designed targeting the conserved L-segment of their genome. Sensitivity was assessed using 10-fold serial dilutions of quantified in vitro DNA samples. Specificity was evaluated by testing different genomic RNA extracted from other representative phleboviruses. The new assay was used for virus detection in sand flies collected in 2012 from the Balearic Archipelago, a touristic hotspot in the Mediterranean.
resultsThe real-time RT-PCR assay exhibited a sensitivity per reaction of 19 copies for Granada and Arrabida, and 16 copies for Massilia. No other related phleboviruses were detected. From the 37 pools of sand fly samples studied from four different Balearic Islands, we detected one positive in the island of Cabrera.
conclusionsTo our knowledge, the method described here is the first real-time RT-PCR designed to detect Granada virus and the related Massilia and Arrabida phleboviruses. The study demonstrated that this is a rapid, robust and reliable assay for the accurate diagnosis of human infections as well as for virus surveillance in vectors.
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