ArticleMicrobial cell factories2020
Escherichia coli metabolism under short-term repetitive substrate dynamics: adaptation and trade-offs.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed.
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- SBR operational strategies for directing mixed microbial cultures toward levulinic acid production using hexoses.Frontiers in bioengineering and biotechnology · 2026Article
- High-throughput profiling of bacterial respiration using the Resipher reveals functional responses to nutrients and antibiotics.Frontiers in microbiology · 2026Article
- Glyceraldehyde-3-phosphate dehydrogenase homologs as bifunctional gatekeepers of metabolic segregation inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Scale-down bioreactors-comparative analysis of configurations.Bioprocess and biosystems engineering · 2025Review
- Strategic targeting of AckA in Mycobacterium tuberculosis using peptide inhibitors.Archives of microbiology · 2025Article
- Adaptations of Gram-Negative and Gram-Positive Probiotic Bacteria in Engineered Living Materials.ACS biomaterials science & engineering · 2025Article
- Controlled burn: interconnections between energy-spilling pathways and metabolic signaling in bacteria.Journal of bacteriology · 2025Review
- Directed Evolution of Microbial Communities in Fermented Foods: Strategies, Mechanisms, and Challenges.Foods (Basel, Switzerland) · 2025Review
- Bacterial clustering amplifies the reshaping of eutrophic plumes around marine particles: A hybrid data-driven model.PLoS computational biology · 2024Article
- Combined oxygen and glucose oscillations distinctly change the transcriptional and physiological state of Escherichia coli.Microbial biotechnology · 2024Article
- Repeated glucose oscillations in high cell-density cultures influence stress-related functions ofPNAS nexus · 2024Article
- Understanding and computational design of genetic circuits of metabolic networks.Essays in biochemistry · 2024Review
- Minimizing acetate formation from overflow metabolism inFrontiers in bioengineering and biotechnology · 2024Article
- A Unique Response Behavior in the Dissolved Oxygen Tension inBioengineering (Basel, Switzerland) · 2023Article
- Optimized Fast Filtration-Based Sampling and Extraction Enables Precise and Absolute Quantification of theMetabolites · 2023Article
- Comparative study of virulence potential, phylogenetic origin, CRISPR-Frontiers in microbiology · 2023Article
- Article
- Metabolic Dynamics in Short- and Long-Term Microgravity in Human Primary Macrophages.International journal of molecular sciences · 2021Article
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Abstract
backgroundMicrobial metabolism is highly dependent on the environmental conditions. Especially, the substrate concentration, as well as oxygen availability, determine the metabolic rates. In large-scale bioreactors, microorganisms encounter dynamic conditions in substrate and oxygen availability (mixing limitations), which influence their metabolism and subsequently their physiology. Earlier, single substrate pulse experiments were not able to explain the observed physiological changes generated under large-scale industrial fermentation conditions.
resultsIn this study we applied a repetitive feast-famine regime in an aerobic Escherichia coli culture in a time-scale of seconds. The regime was applied for several generations, allowing cells to adapt to the (repetitive) dynamic environment. The observed response was highly reproducible over the cycles, indicating that cells were indeed fully adapted to the regime. We observed an increase of the specific substrate and oxygen consumption (average) rates during the feast-famine regime, compared to a steady-state (chemostat) reference environment. The increased rates at same (average) growth rate led to a reduced biomass yield (30% lower). Interestingly, this drop was not followed by increased by-product formation, pointing to the existence of energy-spilling reactions. During the feast-famine cycle, the cells rapidly increased their uptake rate. Within 10 s after the beginning of the feeding, the substrate uptake rate was higher (4.68 μmol/g
conclusionsThe adaptation of the physiology and metabolism of E. coli under substrate dynamics, representative for large-scale fermenters, revealed the existence of several cellular mechanisms coping with stress. Changes in the substrate uptake system, storage potential and energy-spilling processes resulted to be of great importance. These metabolic strategies consist a meaningful step to further tackle reduced microbial performance, observed under large-scale cultivations.
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