ArticleMicrobial cell factories2020
Development of a whole-cell biocatalyst for diisobutyl phthalate degradation by functional display of a carboxylesterase on the surface of Escherichia coli.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 37 citations in OpenAlex.
- Bacterial degradation of aromatic ester pollutants in agro-ecosystems: implications for bioremediation.Applied microbiology and biotechnology · 2026Review
- Synergistic effects of ionic liquid-solvent systems on geniposide cinnamoylation catalyzed by whole-cell biocatalysts.Bioprocess and biosystems engineering · 2026Article
- Engineering bacterial biocatalysts for the degradation of phthalic acid esters.Microbial biotechnology · 2024Review
- Identification and characterisation of moderately thermostable diisobutyl phthalate degrading esterase from a Great Artesian BasinBiotechnology reports (Amsterdam, Netherlands) · 2024Article
- Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly.Microbial cell factories · 2024Article
- Molecular insights into the catalytic mechanism of plasticizer degradation by a monoalkyl phthalate hydrolase.Communications chemistry · 2023Article
- Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display.Bioresources and bioprocessing · 2022Article
- Display of a novel carboxylesterase CarCby on Escherichia coli cell surface for carbaryl pesticide bioremediation.Microbial cell factories · 2022Article
- Phthalate Esters Metabolic StrainMicroorganisms · 2022Article
- Application of Corynebacterium glutamicum engineering display system in three generations of biorefinery.Microbial cell factories · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPhthalic acid esters (PAEs) are widely used as plasticizers or additives during the industrial manufacturing of plastic products. PAEs have been detected in both aquatic and terrestrial environments due to their overuse. Exposure of PAEs results in human health concerns and environmental pollution. Diisobutyl phthalate is one of the main plasticizers in PAEs. Cell surface display of recombinant proteins has become a powerful tool for biotechnology applications. In this current study, a carboxylesterase was displayed on the surface of Escherichia coli cells, for use as whole-cell biocatalyst in diisobutyl phthalate biodegradation.
resultsA carboxylesterase-encoding gene (carEW) identified from Bacillus sp. K91, was fused to the N-terminal of ice nucleation protein (inpn) anchor from Pseudomonas syringae and gfp gene, and the fused protein was then cloned into pET-28a(+) vector and was expressed in Escherichia coli BL21(DE3) cells. The surface localization of INPN-CarEW/or INPN-CarEW-GFP fusion protein was confirmed by SDS-PAGE, western blot, proteinase accessibility assay, and green fluorescence measurement. The catalytic activity of the constructed E. coli surface-displayed cells was determined. The cell-surface-displayed CarEW displayed optimal temperature of 45 °C and optimal pH of 9.0, using p-NPC
conclusionsThis work provides a promising strategy of cost-efficient biodegradation of diisobutyl phthalate for environmental bioremediation by displaying CarEW on the surface of E. coli cells. This approach might also provide a reference in treatment of other different kinds of environmental pollutants by displaying the enzyme of interest on the cell surface of a harmless microorganism.
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