Evidence map›Paper›PMID 32471212›Full record

ArticleInternational journal of molecular sciences2020

Isolation of Human Small Extracellular Vesicles and Tracking of their Uptake by Retinal Pigment Epithelial Cells In Vitro.

Irene C Marcu, Naja Eberhard, Anaïs Yerly, Verena Balmer, Andrew Hemphill, Helga Mogel, Véronique Gaschen, Michael H Stoffel, Jasmin Bluteau

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Irene C MarcuDivision of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
Naja EberhardInstitute of Parasitology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
Anaïs YerlyDivision of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
Verena BalmerInstitute of Parasitology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
Andrew HemphillInstitute of Parasitology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.ORCID 0000-0002-0622-2128
Helga MogelDivision of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
Véronique GaschenDivision of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
Michael H StoffelDivision of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.ORCID 0000-0002-4699-5125
Jasmin BluteauDivision of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
University of Bern · CH

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 310030_184662
6 · The paper itself

Abstract

Small extracellular vesicles (EVs) are among the most frequently investigated EVs and play major roles in intercellular communication by delivering various cargo molecules to target cells. They could potentially represent an alternative delivery strategy to treat ocular toxoplasmosis, a parasitosis affecting the retinal pigment epithelium (RPE). To date, the uptake of human small EVs by RPE cells has never been reported. In this study, we report on the intracellular uptake of fluorescently labelled human urine and fibroblast-derived small EVs by human RPE cells. In summary, both dye-labelled urinary small EVs and small EVs obtained from fibroblasts stably expressing membrane-bound green fluorescent protein were successfully internalized by RPE cells as revealed by immunohistochemistry. In recipient ARPE19 cells, BODIPY-labelled small EVs were found in close vicinity to the parasite

Indexed as

Biological TransportCells, CulturedExtracellular VesiclesHEK293 CellsHumansRetinal Pigment EpitheliumBODIPYEVPKHretinal pigment epitheliumsmall extracellular vesiclesT. gondii

Identifiers

PMID32471212
PMCPMC7313035
OpenAlexW3032522337

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.