Evidence map›Paper›PMID 32471034›Full record

ArticleInternational journal of molecular sciences2020

Affibody-Binding Ligands.

Annalisa Barozzi, R Ashton Lavoie, Kevin N Day, Raphael Prodromou, Stefano Menegatti

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Development of ZFrontiers in immunology · 2025
    Article
  4. Long-Acting Ocular Injectables: Are We Looking In The Right Direction?Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Annalisa BarozziDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
R Ashton LavoieDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
Kevin N DayDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
Raphael ProdromouDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
Stefano MenegattiDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.ORCID 0000-0001-5633-434X

Funding

National Science Foundation 1653590
6 · The paper itself

Abstract

While antibodies remain established therapeutic and diagnostic tools, other protein scaffolds are emerging as effective and safer alternatives. Affibodies in particular are a new class of small proteins marketed as bio-analytic reagents. They feature tailorable binding affinity, low immunogenicity, high tissue permeation, and high expression titer in bacterial hosts. This work presents the development of affibody-binding peptides to be utilized as ligands for their purification from bacterial lysates. Affibody-binding candidates were identified by screening a peptide library simultaneously against two model affibodies (anti-immunoglobulin G (IgG) and anti-albumin) with the aim of selecting peptides targeting the conserved domain of affibodies. An ensemble of homologous sequences identified from screening was synthesized on Toyopearl

Indexed as

Amino Acid SequenceHumansImmunoglobulin GLigandsMolecular Docking SimulationPeptide LibraryPeptidesRecombinant Fusion ProteinsSerum Albumin, HumanTemperatureImmunoglobulin GLigandsPeptide LibraryPeptidesRecombinant Fusion ProteinsSerum Albumin, Humanaffibodyaffinity chromatographybiomanufacturingpeptide ligandsprotein purification

Identifiers

PMID32471034
PMCPMC7312911

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.