ArticlePloS one2020
A simplified method for blood feeding, oral infection, and saliva collection of the dengue vector mosquitoes.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Use of Synthetic Membranes and Bovine Blood in Artificial Feeding Systems of Mosquitoes Improves Feeding Rates: Results from a Systematic Review and Meta-Analysis.The American journal of tropical medicine and hygiene · 2025Pooled it
- Bridging laboratory and field research: Method adjustments to blood feed field-derived Aedes aegypti.PLoS neglected tropical diseases · 2026Article
- Article
- A single-nucleus transcriptomic atlas of the adultbioRxiv : the preprint server for biology · 2025Article
- The artificial meal SkitoSnack does not support reproduction in Culex pipiens (Diptera: Culicidae) mosquitoes.Journal of insect science (Online) · 2025Article
- Characterization of Mosquito Salivary Components.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Comparative analysis of duck Tembusu virus Cluster 1 and Cluster 2.1 inCurrent research in parasitology & vector-borne diseases · 2025Article
- Review
- Article
- Histamine excretion in common indoor and hematophagous arthropods.Journal of medical entomology · 2023Article
- Evaluation of "Caserotek" a low cost and effective artificial blood-feeding device for mosquitoes.PLoS neglected tropical diseases · 2023Article
- Do it yourself: fabricating and evaluating a mosquito (Diptera: Culicidae) blood-feeding device to replace a commercial option.Journal of insect science (Online) · 2023Article
- Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control.Pathogens (Basel, Switzerland) · 2023Review
- 34-kDa salivary protein enhances duck Tembusu virus infectivity in the salivary glands of Aedes albopictus by modulating the innate immune response.Scientific reports · 2023Article
- Engineered Human Tissue as A New Platform for Mosquito Bite-Site Biology Investigations.Insects · 2023Article
- Co-infection of dengue and Zika viruses mutually enhances viral replication in the mosquito Aedes aegypti.Parasites & vectors · 2023Article
- Batch RearingCold Spring Harbor protocols · 2023Article
- Octopamine and tyramine signalling in Aedes aegypti: Molecular characterization and insight into potential physiological roles.PloS one · 2023Article
- A simple non-invasive method to collect soft tick saliva reveals differences inFrontiers in cellular and infection microbiology · 2023Article
- SUMOylation Is Essential for Dengue Virus Replication and Transmission in the MosquitoFrontiers in microbiology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple device using folded Parafilm-M as an artificial blood feeder was designed for studying two important dengue vector mosquitoes, Aedes aegypti and Aedes albopictus. The efficiency of the artificial blood feeder was investigated by comparing the numbers of engorged mosquitoes that fed on the artificial blood feeder versus mice as a live blood source. Significantly more engorged females Aedes aegypti fed on the artificial blood feeder than on mice. In addition, the artificial feeder could serve as a useful apparatus for oral infection via artificial blood meals, and for saliva collection in mosquitoes. Our method enabled us to collect saliva from multiple mosquitoes at once, providing sufficient infected saliva for determination of the virus titer by plaque assay analysis. Our artificial feeder has the advantage that it is simple, inexpensive, and efficient.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.