ArticleAnalytical and bioanalytical chemistry2020
Solid-phase extraction of exosomes from diverse matrices via a polyester capillary-channeled polymer (C-CP) fiber stationary phase in a spin-down tip format.
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Comparison of polyester (PET) capillary-channeled polymer (C-CP) fiber spin-down tips and size exclusion chromatography (SEC) for the isolation of HEK 293-derived extracellular vesicles (EVs).Analytical and bioanalytical chemistry · 2026Article
- Comparison of light scattering-based detection methods for the sizing and number density characterization of extracellular vesicles (EV) isolated from human embryonic kidney (HEK) cell cultures.Analytical and bioanalytical chemistry · 2026Article
- Plant-Derived Nanovesicles: Resolving Conceptual Confusion, Overcoming Isolation Challenges, and Advancing Translational Potential.International journal of nanomedicine · 2026Review
- Plant-derived extracellular vesicles quality control: key process progress and future research directions.Discover nano · 2025Review
- Current progress of plant-derived exosome-like nanovesicles on the regulation of osteoporosis and osteoarthritis.Annals of medicine · 2025Review
- Effects of packing density and adsorption conditions on extracellular vesicle dynamic binding capacities for capillary-channeled polymer (C-CP) fiber columns.Journal of chromatography. A · 2025Article
- Amine-Modified Diatomaceous Earth Syringe Platform (DeSEI) for Efficient and Cost-Effective EV Isolation.International journal of molecular sciences · 2025Article
- Isolation of Urinary Extracellular Vesicles (EVs) via Hydrophobic Interaction Chromatography Using a Nylon-6 Capillary-Channeled Polymer (C-CP) Fiber Column.Journal of separation science · 2025Article
- Isolation and quantification of human urinary exosomes using a Tween-20 elution solvent from polyester, capillary-channeled polymer fiber columns.Analytica chimica acta · 2024Article
- Quantitative Recoveries of Exosomes and Monoclonal Antibodies from Chinese Hamster Ovary Cell Cultures by Use of a Single, Integrated Two-Dimensional Liquid Chromatography Method.Analytical chemistry · 2023Article
- Loading characteristics of streptavidin on polypropylene capillary channeled polymer fibers and capture performance towards biotinylated proteins.Analytical and bioanalytical chemistry · 2023Article
- Rapid isolation and quantification of extracellular vesicles from suspension-adapted human embryonic kidney cells using capillary-channeled polymer fiber spin-down tips.Electrophoresis · 2023Article
- Comparison of the capillary-channeled polymer (C-CP) fiber spin-down tip approach to traditional methods for the isolation of extracellular vesicles from human urine.Analytical and bioanalytical chemistry · 2022Article
- Review
- Milk exosomes: Nature's abundant nanoplatform for theranostic applications.Bioactive materials · 2021Article
- Ruminant Milk-Derived Extracellular Vesicles: A Nutritional and Therapeutic Opportunity?Nutrients · 2021Review
- Rapid separation of blood plasma exosomes from low-density lipoproteins via a hydrophobic interaction chromatography method on a polyester capillary-channeled polymer fiber phase.Analytica chimica acta · 2021Article
- A novel method of high-purity extracellular vesicle enrichment from microliter-scale human serum for proteomic analysis.Electrophoresis · 2021Article
- Flood Control: How Milk-Derived Extracellular Vesicles Can Help to Improve the Intestinal Barrier Function and Break the Gut-Joint Axis in Rheumatoid Arthritis.Frontiers in immunology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Exosomes, a subset of the extracellular vesicle (EV) group of organelles, hold great potential for biomarker detection, therapeutics, disease diagnosis, and personalized medicine applications. The promise and potential of these applications are hindered by the lack of an efficient means of isolation, characterization, and quantitation. Current methods for exosome and EV isolation (including ultracentrifugation, microfiltration, and affinity-based techniques) result in impure recoveries with regard to remnant matrix species (e.g., proteins, genetic material) and are performed on clinically irrelevant time and volume scales. To address these issues, a polyethylene terephthalate (PET) capillary-channeled polymer (C-CP) fiber stationary phase is employed for the solid-phase extraction (SPE) of EVs from various matrices using a micropipette tip-based format. The hydrophobic interaction chromatography (HIC) processing and a spin-down workflow are carried out using a table-top centrifuge. Capture and subsequent elution of intact, biologically active exosomes are verified via electron microscopy and bioassays. The performance of this method was evaluated by capture and elution of exosome standards from buffer solution and three biologically relevant matrices: mock urine, reconstituted non-fat milk, and exosome-depleted fetal bovine serum (FBS). Recoveries were evaluated using UV-Vis absorbance spectrophotometry and ELISA assay. The dynamic binding capacity (50%) for the 1-cm-long (~ 5 μL bed volume) tips was determined using a commercial exosome product, yielding a value of ~ 7 × 10
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.