ArticlePlant methods2020
Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization.
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Quantitative RNA spatial profiling using single-molecule RNA FISH on plant tissue cryosections.Plant communications · 2026Article
- Single-cell transcriptome analysis dissects lncRNA-associated gene networks in Arabidopsis.Plant communications · 2024Article
- A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol.Plant methods · 2023Article
- How to explore what is hidden? A review of techniques for vascular tissue expression profile analysis.Plant methods · 2023Review
- A 3D gene expression atlas of the floral meristem based on spatial reconstruction of single nucleus RNA sequencing data.Nature communications · 2022Article
- Transcriptional regulation of the raffinose family oligosaccharides pathway inFrontiers in plant science · 2022Article
- Mutations in a barley cytochrome P450 gene enhances pathogen induced programmed cell death and cutin layer instability.PLoS genetics · 2021Article
- The 3D Genome: From Structure to Function.International journal of molecular sciences · 2021Review
- Potentials of single-cell genomics in deciphering cellular phenotypes.Current opinion in plant biology · 2021Review
- OMICs, Epigenetics, and Genome Editing Techniques for Food and Nutritional Security.Plants (Basel, Switzerland) · 2021Review
- Bacterial Vector-Borne Plant Diseases: Unanswered Questions and Future Directions.Molecular plant · 2020Review
- Expanded application to plant reproductive tissues of a branched DNA probe-based in situ hybridization method.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn situ analysis of biomarkers such as DNA, RNA and proteins are important for research and diagnostic purposes. At the RNA level, plant gene expression studies rely on qPCR, RNAseq and probe-based in situ hybridization (ISH). However, for ISH experiments poor stability of RNA and RNA based probes commonly results in poor detection or poor reproducibility. Recently, the development and availability of the RNAscope RNA-ISH method addressed these problems by novel signal amplification and background suppression. This method is capable of simultaneous detection of multiple target RNAs down to the single molecule level in individual cells, allowing researchers to study spatio-temporal patterning of gene expression. However, this method has not been optimized thus poorly utilized for plant specific gene expression studies which would allow for fluorescent multiplex detection. Here we provide a step-by-step method for sample collection and pretreatment optimization to perform the RNAscope assay in the leaf tissues of model monocot plant barley. We have shown the spatial distribution pattern of
resultsWe have shown the ubiquitous
conclusionsRNAscope multiplex fluorescent RNA-ISH detection is well described and adapted for animal tissue samples, however due to morphological and structural differences in the plant tissues the standard protocol is deficient and required optimization. Utilizing barley specific
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