ArticleProceedings of the National Academy of Sciences of the United States of America2020
Improved bacterial recombineering by parallelized protein discovery.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed.
- Retention and biosynthesis of diverse nitroarenes in Escherichia coli after combinatorial nitroreductase gene deletions.Nature chemistry · 2026Article
- Adaptation-coupled CRISPRi identifies antibiotic-sensitizing genes leading to a novel plasmid selection marker.Nucleic acids research · 2026Article
- Robust cellular transformations of PET deconstruction products by import of glycol esters.Science advances · 2026Article
- Tvp complexes support formation of the VgrG-PAAR spike during Type VI secretion system assembly.The EMBO journal · 2026Article
- Probing the limits of genetic recoding using multi-omics-guided evolution.Nature communications · 2026Article
- A mechanism for substrate quality control in outer membrane protein assembly.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Oligonucleotide genetics forbioRxiv : the preprint server for biology · 2026Article
- Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering.Nature biotechnology · 2026Article
- Diversity-generating retroelements for programmable targeted hypermutagenesis.Nature biotechnology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Automation and machine learning drive rapid optimization of isoprenol production in Pseudomonas putida.Nature communications · 2025Article
- Structurally related phage helicases trigger type III-A3 retron-mediated anti-phage defense across diverse tailed phage families.Nucleic acids research · 2025Article
- MOSAIC: A Highly Efficient, One-Step Recombineering Approach to Plasmid Editing and Diversification.ACS synthetic biology · 2025Article
- Review
- Continuous multiplexed phage genome editing using recombitrons.Nature biotechnology · 2025Article
- Engineered orthogonal and obligate bacterial commensalism mediated by a non-standard amino acid.Nature microbiology · 2025Article
- An experimental census of retrons for DNA production and genome editing.Nature biotechnology · 2025Article
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- A diverse single-stranded DNA-annealing protein library enables efficient genome editing across bacterial phyla.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors.
Funding
Abstract
Exploiting bacteriophage-derived homologous recombination processes has enabled precise, multiplex editing of microbial genomes and the construction of billions of customized genetic variants in a single day. The techniques that enable this, multiplex automated genome engineering (MAGE) and directed evolution with random genomic mutations (DIvERGE), are however, currently limited to a handful of microorganisms for which single-stranded DNA-annealing proteins (SSAPs) that promote efficient recombineering have been identified. Thus, to enable genome-scale engineering in new hosts, efficient SSAPs must first be found. Here we introduce a high-throughput method for SSAP discovery that we call "serial enrichment for efficient recombineering" (SEER). By performing SEER in
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