ArticlePLoS genetics2020
The domesticated transposase ALP2 mediates formation of a novel Polycomb protein complex by direct interaction with MSI1, a core subunit of Polycomb Repressive Complex 2 (PRC2).
Article in PLoS genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- Active and Unusually Expanded PIF/Harbinger Transposable Elements in the Caenorhabditis inopinata Genome.Genome biology and evolution · 2026Article
- TheInternational journal of molecular sciences · 2026Article
- The dual trxG/PcG protein ULTRAPETALA1 modulates H3K27me3 and directly enhances POLYCOMB REPRESSIVE COMPLEX 2 activity for fine-tuned reproductive transitions.Nature plants · 2026Article
- Deciphering the dynamics of active autonomous terminal inverted repeat transposons in the plant kingdom.aBIOTECH · 2026Article
- Plant mobile domain protein-DNA motif modules counteract Polycomb silencing to stabilize gene expression.Nature plants · 2025Article
- Factors determining chromosomal localization of transposable elements in plants.Plant biology (Stuttgart, Germany) · 2025Review
- Molecular Mimicry of Transposable Elements in Plants.Plant & cell physiology · 2025Review
- Molecular dissection of the genetic architecture of phenology underlying Lupinus hispanicus early flowering and adaptation to winter- or spring sowing.Scientific reports · 2025Article
- Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.Nature communications · 2024Article
- Article
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- Expression of OsHARBI1-1 enhances the tolerance of Arabidopsis thaliana to cadmium.BMC plant biology · 2023Article
- Functional dissection and assembly of a small, newly evolved, W chromosome-specific genomic region of the African clawed frog Xenopus laevis.PLoS genetics · 2023Article
- Genome-Wide Identification of WD40 Proteins inGenes · 2023Article
- Transposase expression, element abundance, element size, and DNA repair determine the mobility and heritability ofFrontiers in cell and developmental biology · 2023Article
- Recurrent co-domestication of PIF/Harbinger transposable element proteins in insects.Mobile DNA · 2022Article
- Single-nucleus RNA-seq reveals that MBD5, MBD6, and SILENZIO maintain silencing in the vegetative cell of developing pollen.Cell reports · 2022Article
- The neurodevelopmental gene MSANTD2 belongs to a gene family formed by recurrent molecular domestication of Harbinger transposons at the base of vertebrates.Molecular biology and evolution · 2022Article
- The Harbinger transposon-derived gene PANDA epigenetically coordinates panicle number and grain size in rice.Plant biotechnology journal · 2022Article
- Diversity ofAnimals : an open access journal from MDPI · 2022Article
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21 authors at 7 institutions in 5 countries.
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Abstract
A large fraction of plant genomes is composed of transposable elements (TE), which provide a potential source of novel genes through "domestication"-the process whereby the proteins encoded by TE diverge in sequence, lose their ability to catalyse transposition and instead acquire novel functions for their hosts. In Arabidopsis, ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 (ALP1) arose by domestication of the nuclease component of Harbinger class TE and acquired a new function as a component of POLYCOMB REPRESSIVE COMPLEX 2 (PRC2), a histone H3K27me3 methyltransferase involved in regulation of host genes and in some cases TE. It was not clear how ALP1 associated with PRC2, nor what the functional consequence was. Here, we identify ALP2 genetically as a suppressor of Polycomb-group (PcG) mutant phenotypes and show that it arose from the second, DNA binding component of Harbinger transposases. Molecular analysis of PcG compromised backgrounds reveals that ALP genes oppose silencing and H3K27me3 deposition at key PcG target genes. Proteomic analysis reveals that ALP1 and ALP2 are components of a variant PRC2 complex that contains the four core components but lacks plant-specific accessory components such as the H3K27me3 reader LIKE HETEROCHROMATION PROTEIN 1 (LHP1). We show that the N-terminus of ALP2 interacts directly with ALP1, whereas the C-terminus of ALP2 interacts with MULTICOPY SUPPRESSOR OF IRA1 (MSI1), a core component of PRC2. Proteomic analysis reveals that in alp2 mutant backgrounds ALP1 protein no longer associates with PRC2, consistent with a role for ALP2 in recruitment of ALP1. We suggest that the propensity of Harbinger TE to insert in gene-rich regions of the genome, together with the modular two component nature of their transposases, has predisposed them for domestication and incorporation into chromatin modifying complexes.
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