ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2020
Lipid droplets disrupt mechanosensing in human hepatocytes.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Hepatocyte PIEZO1 Negatively Regulates Lipogenesis and Ameliorates MASLD by Sensing Membrane Tension and Activating AMPK.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The Emerging Role of Mechanobiology in Connecting Metabolic and Cardiovascular Diseases: From Fundamentals to Future Therapies.Biomedicines · 2026Review
- Engineered Hydrogels for Organoid Models of Human Nonalcoholic Fatty Liver Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Elongation factor 1A1 inhibition elicits changes in lipid droplet size, the bulk transcriptome, and cell type-associated gene expression in MASLD mouse liver.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Mechanobiology in Metabolic Dysfunction-Associated Steatotic Liver Disease and Obesity.Current issues in molecular biology · 2024Review
- Mechanobiology of Adipocytes.Biology · 2024Review
- Mechanobiology of portal hypertension.JHEP reports : innovation in hepatology · 2023Review
- The Coumarin-Derivative Esculetin Protects against Lipotoxicity in Primary Rat Hepatocytes via Attenuating JNK-Mediated Oxidative Stress and Attenuates Free Fatty Acid-Induced Lipid Accumulation.Antioxidants (Basel, Switzerland) · 2023Article
- Lipophilic statins inhibit YAP coactivator transcriptional activity in HCC cells through Rho-mediated modulation of actin cytoskeleton.American journal of physiology. Gastrointestinal and liver physiology · 2023Article
- Local fat content determines global and local stiffness in livers with simple steatosis.FASEB bioAdvances · 2023Article
- Lipid droplets are intracellular mechanical stressors that impair hepatocyte function.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Age-dependent nuclear lipid droplet deposition is a cellular hallmark of aging in Caenorhabditis elegans.Aging cell · 2023Article
- Methodological advancements in organ-specific ectopic lipid quantitative characterization: Effects of high fat diet on muscle and liver intracellular lipids.Molecular metabolism · 2023Article
- The immune and metabolic treatment approach of using testosterone on mice models of liver injury.Frontiers in pharmacology · 2023Article
- Durotaxis: the mechanical control of directed cell migration.The FEBS journal · 2022Review
- Article
- Remodeling of Mitochondrial Plasticity: The Key Switch from NAFLD/NASH to HCC.International journal of molecular sciences · 2021Review
- Endomembranes: Unsung Heroes of Mechanobiology?Frontiers in bioengineering and biotechnology · 2020Review
- Liver fibrosis: Our evolving understanding.Clinical liver diseaseReview
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Hepatocellular carcinoma (HCC) is the fourth-leading cause of cancer death in the world. Although most cases occur in stiff, cirrhotic livers, and stiffness is a significant risk factor, HCC can also arise in noncirrhotic livers in the setting of nonalcoholic fatty liver disease (NAFLD). We hypothesized that lipid droplets in NAFLD might apply mechanical forces to the nucleus, functioning as mechanical stressors akin to stiffness. We investigated the effect of lipid droplets on cellular mechanosensing and found that primary human hepatocytes loaded with the fatty acids oleate and linoleate exhibited decreased stiffness-induced cell spreading and disrupted focal adhesions and stress fibers. The presence of large lipid droplets in hepatocytes resulted in increased nuclear localization of the mechano-sensor Yes-associated protein (YAP). In cirrhotic livers from patients with NAFLD, hepatocytes filled with large lipid droplets showed significantly higher nuclear localization of YAP as compared with cells with small lipid droplets. This work suggests that lipid droplets induce a mechanical signal that disrupts the ability of the hepatocyte to sense its underlying matrix stiffness and that the presence of lipid droplets can induce intracellular mechanical stresses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.