Evidence map›Paper›PMID 32462598›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

MALDI Mass Spectrometry Imaging of Peptides in Medicago truncatula Root Nodules.

Caitlin Keller, Erin Gemperline, Lingjun Li

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. MALDI spatial proteomics: a mini review of approaches and techniques.Analytical methods : advancing methods and applications · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Caitlin KellerDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
Erin GemperlineDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
Lingjun LiDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, USA. lingjun.li@wisc.edu.
University of Wisconsin–Madison · US

Funding

Mass Spectrometric Studies of Neuropeptides in FeedingR01DK071801 · NIDDK · UNIVERSITY OF WISCONSIN-MADISON · PI LINGJUN LI · 2006 to 2026
$6.7M
Acquisition of a High-Field Dual Source FTICR-MS for Pharmaceutical ResearchS10RR029531 · NCRR · UNIVERSITY OF WISCONSIN-MADISON · PI LI, LINGJUN · 2011 to 2011
$2.1M
NCRR NIH HHS S10 RR029531NIDDK NIH HHS R01 DK071801
6 · The paper itself

Abstract

Mass spectrometry imaging is routinely used to visualize the distributions of biomolecules in tissue sections. In plants, mass spectrometry imaging of metabolites is more often conducted, but the imaging of larger molecules is less frequently performed despite the importance of proteins and endogenous peptides to the plant. Here, we describe a matrix-assisted laser desorption/ionization mass spectrometry imaging method for the imaging of peptides in Medicago truncatula root nodules. Sample preparation steps including embedding in gelatin, sectioning, and matrix application are described. The method described is employed to determine the spatial distribution of hundreds of peptide peaks.

Indexed as

MetabolomeMedicago truncatulaMolecular ImagingPeptide FragmentsPlant ProteinsRoot Nodules, PlantSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationPeptide FragmentsPlant ProteinsMALDIMass spectrometry imagingMedicago truncatulaMSIPeptidesRoot nodules

Identifiers

PMID32462598
PMCPMC7430052
OpenAlexW3030882244

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.