ArticleProceedings of the National Academy of Sciences of the United States of America2020
Glycosaminoglycans accelerate biomimetic collagen mineralization in a tissue-based in vitro model.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
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- Effects and Properties of Deproteinizing Methods in Dentin: A Comprehensive Narrative Review.Journal of functional biomaterials · 2026Review
- Harnessing polysaccharide-mediated biomineralization for advanced bone tissue engineering.Materials today. Bio · 2026Review
- Human Endometrial Extracellular Matrix Hydrogel Facilitated Endometrial Mesenchymal Stem Cells for Endometrial Regeneration.Advanced healthcare materials · 2026Article
- Development and optimization of electrophoretically deposited octacalcium phosphate-collagen film as bone analogues.Regenerative biomaterials · 2026Article
- Mineral Content and Extracellular Matrix Protein Expression in Mouse Growth Plates During Epiphyseal Fusion: An Observational Study.Calcified tissue international · 2025Article
- Calcium phosphate nanoclusters modify periodontium remodeling and minimize orthodontic relapse.Biomaterials · 2025Article
- Multifunctional DNA-Collagen Biomaterials: Developmental Advances and Biomedical Applications.ACS biomaterials science & engineering · 2025Review
- A new ex vivo human model of osteoarthritis cartilage calcification.Rheumatology (Oxford, England) · 2025Article
- Mechanistic studies of chondroitin sulfate/dermatan sulfate isolated from freshwater fish discards on osteogenesis in MC3T3-E1 cells.Glycoconjugate journal · 2025Article
- Unraveling the Mechanisms of Hypertrophy-Induced Matrix Mineralization and Modifications in Articular Chondrocytes.Calcified tissue international · 2024Article
- Calcium phosphate nanoclusters modify periodontium remodeling and minimize orthodontic relapse.bioRxiv : the preprint server for biology · 2024Article
- Towards Polycaprolactone-Based Scaffolds for Alveolar Bone Tissue Engineering: A Biomimetic Approach in a 3D Printing Technique.International journal of molecular sciences · 2023Review
- Article
- Periodontal regeneration: Lessons from the periodontal ligament-cementum junction in diverse animal models.Frontiers in dental medicine · 2023Article
- Stability and remineralization of proteoglycan-infused dentin substrate.Dental materials : official publication of the Academy of Dental Materials · 2021Article
- Mineralized DNA-collagen complex-based biomaterials for bone tissue engineering.International journal of biological macromolecules · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian teeth are attached to the jawbone through an exquisitely controlled mineralization process: unmineralized collagen fibers of the periodontal ligament anchor directly into the outer layer of adjoining mineralized tissues (cementum and bone). The sharp interface between mineralized and nonmineralized collagenous tissues makes this an excellent model to study the mechanisms by which extracellular matrix macromolecules control collagen mineralization. While acidic phosphoproteins, localized in the mineralized tissues, play key roles in control of mineralization, the role of glycosaminoglycans (GAGs) is less clear. As several proteoglycans are found only in the periodontal ligament, it has been hypothesized that these inhibit mineralization of collagen in this tissue. Here we used an in vitro model based on remineralization of mouse dental tissues to determine the role of matrix GAGs in control of mineralization. GAGs were selectively removed from demineralized mouse periodontal sections via enzymatic digestion. Proteomic analysis confirmed that enzymatic GAG removal does not significantly alter protein content. Analysis of remineralized tissue sections by transmission electron microscopy (TEM) shows that GAG removal reduced the rate of remineralization in mineralized tissues compared to the untreated control, while the ligament remained unmineralized. Protein removal with trypsin also reduced the rate of mineralization, but to a lesser extent than GAG removal, despite a much larger effect on protein content. These results indicate that GAGs promote mineralization in mineralized dental tissues rather than inhibiting mineral formation in the ligament, which may have broader implications for understanding control of collagen mineralization in connective tissues.
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