Evidence map›Paper›PMID 32460715›Full record

ArticleBMC cancer2020

A novel pancreatic tumour and stellate cell 3D co-culture spheroid model.

K J Norberg, X Liu, C Fernández Moro, C Strell, S Nania, M Blümel, A Balboni, B Bozóky, R L Heuchel, J M Löhr

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

K J NorbergPancreas Cancer Research Lab, Department of Clinical Intervention and Technology (CLINTEC), Karolinska Institutet, Novum, floor 6, room 613, SE-141 86, Stockholm, Sweden.
X LiuPancreas Cancer Research Lab, Department of Clinical Intervention and Technology (CLINTEC), Karolinska Institutet, Novum, floor 6, room 613, SE-141 86, Stockholm, Sweden.
C Fernández MoroDepartment of Laboratory Medicine (LabMed), Division of Pathology, Karolinska Institutet, Stockholm, Sweden.
C StrellDepartment of Cancer, Division of Upper GI, Karolinska University Hospital, Stockholm, Sweden.
S NaniaPancreas Cancer Research Lab, Department of Clinical Intervention and Technology (CLINTEC), Karolinska Institutet, Novum, floor 6, room 613, SE-141 86, Stockholm, Sweden.
M BlümelPancreas Cancer Research Lab, Department of Clinical Intervention and Technology (CLINTEC), Karolinska Institutet, Novum, floor 6, room 613, SE-141 86, Stockholm, Sweden.
A BalboniPancreas Cancer Research Lab, Department of Clinical Intervention and Technology (CLINTEC), Karolinska Institutet, Novum, floor 6, room 613, SE-141 86, Stockholm, Sweden.
B BozókyDepartment of Laboratory Medicine (LabMed), Division of Pathology, Karolinska Institutet, Stockholm, Sweden.
R L HeuchelPancreas Cancer Research Lab, Department of Clinical Intervention and Technology (CLINTEC), Karolinska Institutet, Novum, floor 6, room 613, SE-141 86, Stockholm, Sweden. Rainer.heuchel@ki.se.
J M LöhrPancreas Cancer Research Lab, Department of Clinical Intervention and Technology (CLINTEC), Karolinska Institutet, Novum, floor 6, room 613, SE-141 86, Stockholm, Sweden.

Funding

Cancerfonden CAN2013/780, CAN2017/615China Scholarship Council 201700260279FP7-Health ID: 256974Radiumhemmets Forskningsfonder 111252, 131163Vetenskapsrådet K2013-67X22322-01-3
6 · The paper itself

Abstract

backgroundPancreatic ductal adenocarcinoma is a devastating disease with poor outcome, generally characterized by an excessive stroma component. The purpose of this study was to develop a simple and reproducible in vitro 3D-assay employing the main constituents of pancreatic ductal adenocarcinoma, namely pancreatic stellate and cancer cells.

methodA spheroid assay, directly co-culturing human pancreatic stellate cells with human pancreatic tumour cells in 3D was established and characterized by electron microscopy, immunohistochemistry and real-time RT-PCR. In order to facilitate the cell type-specific crosstalk analysis by real-time RT-PCR, we developed a novel in vitro 3D co-culture model, where the participating cell types were from different species, human and mouse, respectively. Using species-specific PCR primers, we were able to investigate the crosstalk between stromal and cancer cells without previous cell separation and sorting.

resultsWe found clear evidence for mutual influence, such as increased proliferation and a shift towards a more mesenchymal phenotype in cancer cells and an activation of pancreatic stellate cells towards the myofibroblast phenotype. Using a heterospecies approach, which we coined virtual sorting, confirmed the findings we made initially in the human-human spheroids.

conclusionsWe developed and characterized different easy to set up 3D models to investigate the crosstalk between cancer and stroma cells for pancreatic cancer.

Indexed as

Carcinoma, Pancreatic DuctalCell CommunicationCell Line, TumorCell ProliferationCoculture TechniquesHumansImmunohistochemistryMicroscopy, ElectronPancreatic NeoplasmsPancreatic Stellate CellsPhenotypeReal-Time Polymerase Chain ReactionSpheroids, Cellular3D cell cultureGene expressionPDACReal time RT-PCRVirtual sorting

Identifiers

PMID32460715
PMCPMC7251727

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.