ArticleBMC plant biology2020
LcMYB4, an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis.
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 32 citations in OpenAlex.
- Dynamic and non-additive gene regulation shapes maize responses to simultaneous salt and cold stress.Functional & integrative genomics · 2026Article
- Holistic overview of regulatory networks governing seed dormancy and germination in plants.Science China. Life sciences · 2026Review
- A novel natural variation in the promoter of TaDHN4D1 confers salt tolerance in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- The Combination of Physiological and Transcriptomic Approaches Reveals New Insights into the Molecular Mechanisms ofInternational journal of molecular sciences · 2025Article
- Transcriptomic characterization of Trichoderma harzianum T34 primed tomato plants: assessment of biocontrol agent induced host specific gene expression and plant growth promotion.BMC plant biology · 2023Article
- Integrated Transcriptomic and Metabolomics Analyses Reveal Molecular Responses to Cold Stress in Coconut (International journal of molecular sciences · 2023Article
- Transcriptome Analysis Reveals Brassinolide Signaling Pathway Control of Foxtail Millet Seedling Starch and Sucrose Metabolism under Freezing Stress, with Implications for Growth and Development.International journal of molecular sciences · 2023Article
- Overexpression ofInternational journal of molecular sciences · 2023Article
- Genome-wide transcriptional profiling provides clues to molecular mechanisms underlying cold tolerance in chickpea.Scientific reports · 2023Article
- Article
- Meta-analysis of the effect of expression of MYB transcription factor genes on abiotic stress.PeerJ · 2021Article
- Integrating transcriptome and metabolome analyses of the response to cold stress in pumpkin (Cucurbita maxima).PloS one · 2021Article
- From the Outside to the Inside: New Insights on the Main Factors That Guide Seed Dormancy and Germination.Genes · 2020Review
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundSheepgrass (Leymus chinensis (Trin.) Tzvel) is a perennial forage grass that can survive extreme freezing winters (- 47.5 °C) in China. In this study, we isolated an unknown function MYB transcription factor gene, LcMYB4, from sheepgrass. However, the function of LcMYB4 and its homologous genes has not been studied in other plants.
resultsThe expression of the LcMYB4 gene was upregulated in response to cold induction, and the LcMYB4 fusion protein was localized in the nucleus, with transcriptional activation activity. Biological function analysis showed that compared with WT plants, LcMYB4-overexpressing Arabidopsis presented significantly increased chilling and freezing tolerance as evidenced by increased germination rate, survival rate, and seed setting rate under conditions of low temperature stress. Furthermore, LcMYB4-overexpressing plants showed increased soluble sugar content, leaf chlorophyll content and superoxide dismutase activity but decreased malondialdehyde (MDA) under chilling stress. Moreover, the expression of the CBF1, KIN1, KIN2 and RCI2A genes were significantly upregulated in transgenic plants with chilling treatment. These results suggest that LcMYB4 overexpression increased the soluble sugar content and cold-inducible gene expression and alleviated oxidative damage and membrane damage, resulting in enhanced cold resistance in transgenic plants. Interestingly, our results showed that the LcMYB4 protein interacts with fructose-1,6-bisphosphate aldolase protein1 (LcFBA1) and that the expression of the LcFBA1 gene was also upregulated during cold induction in sheepgrass, similar to LcMYB4.
conclusionOur findings suggest that LcMYB4 encodes MYB transcription factor that plays a positive regulatory role in cold stress.
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