ArticleJournal of experimental botany2020
Oxford Nanopore sequencing: new opportunities for plant genomics?
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Pooled it
- Contrasting regulation of protein-coding genes and lncRNA homeologs in allotetraploid Coffea arabica.Planta · 2026Article
- Structural Variation and Its Roles in Plant Genomes.Plants (Basel, Switzerland) · 2026Review
- Multiomics analysis ofHorticulture research · 2026Article
- Tomato and its relatives are breaking the genomics barriers.Journal of experimental botany · 2025Review
- Genomics-assisted breeding for designing salinity-smart future crops.Plant biotechnology journal · 2025Review
- Chromosome-level assembly ofBreeding science · 2025Article
- Unravelling the Chloroplast Genome of the Kazakh Apricot (Plants (Basel, Switzerland) · 2025Article
- High-throughput sequencing: a breakthrough in molecular diagnosis for precision medicine.Functional & integrative genomics · 2025Review
- PubPlant - a continuously updated online resource for sequenced and published plant genomes.Frontiers in plant science · 2025Article
- Chromosome-Level Genome Assembly of the Australian Rainforest Tree Rhodamnia argentea (Malletwood).Genome biology and evolution · 2024Article
- Snow alga Sanguina aurantia as revealed through de novo genome assembly and annotation.G3 (Bethesda, Md.) · 2024Article
- An evaluation of nucleic acid-based molecular methods for the detection of plant viruses: a systematic review.Virusdisease · 2024Review
- Nanopore-based glycan sequencing: state of the art and future prospects.Chemical science · 2024Review
- Genomic resources for the Yellowfin tuna Thunnus albacares.Molecular biology reports · 2024Article
- Striking variation in chromosome structure withinFrontiers in plant science · 2024Article
- Article
- Deciphering the genetic architecture of fruit color in strawberry.Journal of experimental botany · 2023Review
- Recent Advances in Metagenomic Approaches, Applications, and Challenge.Current microbiology · 2023Review
- The Progression in Developing Genomic Resources for Crop Improvement.Life (Basel, Switzerland) · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA sequencing was dominated by Sanger's chain termination method until the mid-2000s, when it was progressively supplanted by new sequencing technologies that can generate much larger quantities of data in a shorter time. At the forefront of these developments, long-read sequencing technologies (third-generation sequencing) can produce reads that are several kilobases in length. This greatly improves the accuracy of genome assemblies by spanning the highly repetitive segments that cause difficulty for second-generation short-read technologies. Third-generation sequencing is especially appealing for plant genomes, which can be extremely large with long stretches of highly repetitive DNA. Until recently, the low basecalling accuracy of third-generation technologies meant that accurate genome assembly required expensive, high-coverage sequencing followed by computational analysis to correct for errors. However, today's long-read technologies are more accurate and less expensive, making them the method of choice for the assembly of complex genomes. Oxford Nanopore Technologies (ONT), a third-generation platform for the sequencing of native DNA strands, is particularly suitable for the generation of high-quality assemblies of highly repetitive plant genomes. Here we discuss the benefits of ONT, especially for the plant science community, and describe the issues that remain to be addressed when using ONT for plant genome sequencing.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.