Evidence map›Paper›PMID 32459554›Full record

ArticleIslets2020

An islet maturation media to improve the development of young porcine islets during in vitro culture.

Hien Lau, Nicole Corrales, Samuel Rodriguez, Colleen Luong, Frank Zaldivar, Michael Alexander, Jonathan R T Lakey

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Islets, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Hien LauDepartment of Surgery, University of California, Irvine , Orange, CA, USA.ORCID 0000-0002-5575-0875
Nicole CorralesDepartment of Surgery, University of California, Irvine , Orange, CA, USA.
Samuel RodriguezDepartment of Surgery, University of California, Irvine , Orange, CA, USA.
Colleen LuongDepartment of Surgery, University of California, Irvine , Orange, CA, USA.
Frank ZaldivarDepartment of Pediatrics, Pediatric Exercise and Genomics Research Center, University of California, Irvine , Irvine, CA, USA.
Michael AlexanderDepartment of Surgery, University of California, Irvine , Orange, CA, USA.
Jonathan R T LakeyDepartment of Surgery, University of California, Irvine , Orange, CA, USA.
University of California, Irvine · US

Funding

University of California Health Participation in the National COVID Cohort Collaborative (N3C)UL1TR001414 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI COOPER, DAN M, VILAIN, ERIC J. · 2015 to 2023
$35.1M
NCATS NIH HHS UL1 TR001414
6 · The paper itself

Abstract

backgroundThe use of pancreata from pre-weaned piglets has the potential to serve as an unlimited alternative source of islets for clinical xenotransplantation. As pre-weaned porcine islets (PPIs) are immature and require prolonged culture, we developed an islet maturation media (IMM) and evaluated its effect on improving the quantity and quality of PPIs over 14 days of culture.

methodsPPIs were isolated from the pancreata of pre-weaned Yorkshire piglets (8-15 days old). Each independent islet isolation was divided for culture in either control Ham's F-10 media (n = 5) or IMM (n = 5) for 14 days. On day 3, 7 and 14 of culture, islets were assessed for islet yield, isolation index, viability, insulin content, endocrine cellular composition, differentiation of beta cells, and insulin secretion during glucose stimulation.

resultsIn comparison to control islets, culturing PPIs in IMM significantly increased islet yield. PPIs cultured in IMM also maintained a stable isolation index and viability throughout 14 days of culture. The insulin content, endocrine cellular composition, and differentiation of beta cells were significantly improved in PPIs cultured in IMM, which subsequently augmented their insulin secretory capacity in response to glucose challenge compared to control islets.

conclusionsCulturing PPIs in IMM increases islet yield, isolation index, viability, insulin content, endocrine cellular composition, differentiation of endocrine progenitor cells toward beta cells, and insulin secretion. Due to the improved islet quantity and quality after

Indexed as

Culture MediaAnimalsAnimals, NewbornCell DifferentiationCells, CulturedCell SurvivalFlow CytometryInsulinIslets of LangerhansSwineCulture MediaInsulinculture mediain vitro cultureislet culturePre-weaned porcine isletsType 1 diabetes

Identifiers

PMID32459554
PMCPMC7527017
OpenAlexW3031640839

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.