Evidence map›Paper›PMID 32459360›Full record

ArticleThe Journal of infectious diseases2020

Evolution of Antimalarial Drug Resistance Markers in the Reservoir of Plasmodium falciparum Infections in the Upper East Region of Ghana.

Charles A Narh, Anita Ghansah, Michael F Duffy, Shazia Ruybal-Pesántez, Christiana O Onwona, Abraham R Oduro, Kwadwo A Koram, Karen P Day, Kathryn E Tiedje

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 19 citations in OpenAlex.

  1. Assessing the threat of artemisinin resistance: seasonal and ecological patterns ofFrontiers in cellular and infection microbiology · 2026
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  9. Bibliometric analysis of antimalarial drug resistance.Frontiers in cellular and infection microbiology · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Charles A NarhNoguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.
Anita GhansahNoguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.
Michael F DuffySchool of BioSciences, The University of Melbourne, Bio21 Molecular Science and Biotechnology Institute, Melbourne, Australia.
Shazia Ruybal-PesántezSchool of BioSciences, The University of Melbourne, Bio21 Molecular Science and Biotechnology Institute, Melbourne, Australia.
Christiana O OnwonaNoguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.
Abraham R OduroNavrongo Health Research Centre, Ghana Health Service, Navrongo, Ghana.
Kwadwo A KoramNoguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.
Karen P DaySchool of BioSciences, The University of Melbourne, Bio21 Molecular Science and Biotechnology Institute, Melbourne, Australia.
Kathryn E TiedjeSchool of BioSciences, The University of Melbourne, Bio21 Molecular Science and Biotechnology Institute, Melbourne, Australia.
The University of Melbourne · AUUniversity of Ghana · GHNavrongo Health Research Centre · GH

Funding

Temporal strain structure and response to interventions in a high endemicity region of Plasmodium falciparum R01AI149779 · NIAID · UNIVERSITY OF CHICAGO · PI DAY, KAREN PATRICIA, KORAM, KWADWO ANSAH · 2019 to 2023
$3.0M
The impact of seasonality and vector control on the population structure of Plasmodium falciparumR01TW009670 · FIC · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAY, KAREN PATRICIA, KORAM, KWADWO ANSAH · 2013 to 2016
$1.8M
FIC NIH HHS R01 TW009670NIAID NIH HHS R01 AI149779
6 · The paper itself

Abstract

backgroundThe majority of Plasmodium falciparum infections, constituting the reservoir in all ages, are asymptomatic in high-transmission settings in Africa. The role of this reservoir in the evolution and spread of drug resistance was explored.

methodsPopulation genetic analyses of the key drug resistance-mediating polymorphisms were analyzed in a cross-sectional survey of asymptomatic P. falciparum infections across all ages in Bongo District, Ghana.

resultsSeven years after the policy change to artemisinin-based combination therapies in 2005, the pfcrt K76 and pfmdr1 N86 wild-type alleles have nearly reached fixation and have expanded via soft selective sweeps on multiple genetic backgrounds. By constructing the pfcrt-pfmdr1-pfdhfr-pfdhps multilocus haplotypes, we found that the alleles at these loci were in linkage equilibrium and that multidrug-resistant parasites have not expanded in this reservoir. For pfk13, 32 nonsynonymous mutations were identified; however, none were associated with artemisinin-based combination therapy resistance.

conclusionsThe prevalence and selection of alleles/haplotypes by antimalarials were similar to that observed among clinical cases in Ghana, indicating that they do not represent 2 subpopulations with respect to these markers. Thus, the P. falciparum reservoir in all ages can contribute to the maintenance and spread of antimalarial resistance.

Indexed as

AdolescentAdultAgedAged, 80 and overAllelesAntimalarialsArtemisininsATP-Binding Cassette, Sub-Family C ProteinsChildChild, PreschoolCross-Sectional StudiesDrug ResistanceFemaleGenetics, PopulationGenetic VariationGenotypeAntimalarialsartemisininArtemisininsATP-Binding Cassette, Sub-Family C ProteinsMdr1 protein, Plasmodium falciparumMembrane Transport ProteinsPfCRT protein, Plasmodium falciparumProtozoan Proteinsantimalarialsartemisinin-based combination therapiesasymptomatic reservoirdrug resistanceGhanamalariaPlasmodium falciparumpopulation genetics

Identifiers

PMID32459360
PMCPMC7982568
OpenAlexW3030594277

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.