ArticleMolecular microbiology2020
Inhibition of Escherichia coli chromosome replication by rifampicin treatment or during the stringent response is overcome by de novo DnaA protein synthesis.
Article in Molecular microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Risk factors for drug-induced liver injury in tuberculosis patients: a meta-analysis and systematic review.Frontiers in medicine · 2026Pooled it
- Bacterial growth rate in populations and single cells.Nature microbiology · 2026Review
- Synergistic activity of rifampicin and polymyxin B against intracellular Gram-negative ESKAPE pathogens involves bacterial membrane alterations and enhanced oxidative damages.Antimicrobial agents and chemotherapy · 2026Article
- The chromosomal origin of replication as the basis for the spatio-temporal biology of bacteria.Frontiers in microbiology · 2026Review
- A novel membrane stress response that blocks chromosomal replication by targeting the DnaA initiator via the ClpP protease.Journal of bacteriology · 2025Article
- Stress-induced proteins enhance thermal endurance in soil bacterium Priestia aryabhattai strain PSK.N2.Antonie van Leeuwenhoek · 2025Article
- Review
- Regulatory elements coordinating initiation of chromosome replication to theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Article
- Arresting chromosome replication upon energy starvation in Escherichia coli.Current genetics · 2021Review
- Targeted chromosomal Escherichia coli:dnaB exterior surface residues regulate DNA helicase behavior to maintain genomic stability and organismal fitness.PLoS genetics · 2021Article
- Antisense inhibition of the Escherichia coli NrdAB aerobic ribonucleotide reductase is bactericidal due to induction of DNA strand breaks.The Journal of antimicrobial chemotherapy · 2021Article
- The stringent response and physiological roles of (pp)pGpp in bacteria.Nature reviews. Microbiology · 2021Review
- Energy Starvation Induces a Cell Cycle Arrest inFrontiers in molecular biosciences · 2021Article
- Transcriptional Activity of the Bacterial Replication Initiator DnaA.Frontiers in microbiology · 2021Review
- Inhibition of Escherichia coli chromosome replication by rifampicin treatment or during the stringent response is overcome by de novo DnaA protein synthesis.Molecular microbiology · 2020Article
- Bacterial Chromosome Replication and DNA Repair During the Stringent Response.Frontiers in microbiology · 2020Review
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2 authors.
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Abstract
Initiation of Escherichia coli chromosome replication is controlled by the DnaA initiator protein. Both rifampicin-mediated inhibition of transcription and ppGpp-induced changes in global transcription stops replication at the level of initiation. Here, we show that continued DnaA protein synthesis allows for replication initiation both during the rifampicin treatment and during the stringent response when the ppGpp level is high. A reduction in or cessation of de novo DnaA synthesis, therefore, causes the initiation arrest in both cases. In accordance with this, inhibition of translation with chloramphenicol also stops initiations. The initiation arrest caused by rifampicin was faster than that caused by chloramphenicol, despite of the latter inhibiting DnaA accumulation immediately. During chloramphenicol treatment transcription is still ongoing and we suggest that transcriptional events in or near the origin, that is, transcriptional activation, can allow for a few extra initiations when DnaA becomes limiting. We suggest, for both rifampicin treated cells and for cells accumulating ppGpp, that a turn-off of initiation from oriC requires a stop in de novo DnaA synthesis and that an additional lack of transcriptional activation enhances this process, that is, leads to a faster initiation stop.
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