ArticleBlood2020
Multispecies RNA tomography reveals regulators of hematopoietic stem cell birth in the embryonic aorta.
Article in Blood, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- RNA tomography reveals spatial gene expression maps of Arabidopsis thaliana roots infected with Heterodera schachtii.The New phytologist · 2026Article
- Widespread presence of bone marrow-like hematopoietic stem cell niche in invertebrate skeletons.Science advances · 2025Article
- tomoseqr: A Bioconductor package for spatial reconstruction and visualization of 3D gene expression patterns based on RNA tomography.PloS one · 2025Article
- NR4A1 and NR4A2 orphan nuclear receptors regulate endothelial-to-hematopoietic transition in mouse hematopoietic stem cell specification.Development (Cambridge, England) · 2024Article
- The molecular and cellular hematopoietic stem cell specification niche.Experimental hematology · 2024Review
- A subset of megakaryocytes regulates development of hematopoietic stem cell precursors.The EMBO journal · 2024Article
- A multistep computational approach reveals a neuro-mesenchymal cell population in the embryonic hematopoietic stem cell niche.Development (Cambridge, England) · 2024Article
- Autophagy regulates the maturation of hematopoietic precursors in the embryo.Nature communications · 2024Article
- An interactive resource of molecular signalling in the developing human haematopoietic stem cell niche.Development (Cambridge, England) · 2023Article
- The emerging Janus face of SVEP1 in development and disease.Trends in molecular medicine · 2023Review
- Meis1 establishes the pre-hemogenic endothelial state prior to Runx1 expression.Nature communications · 2023Article
- De Novo Generation of Human Hematopoietic Stem Cells from Pluripotent Stem Cells for Cellular Therapy.Cells · 2023Review
- p57Kip2 regulates embryonic blood stem cells by controlling sympathoadrenal progenitor expansion.Blood · 2022Article
- PDGFRβCell reports · 2022Article
- Engineering a niche supporting hematopoietic stem cell development using integrated single-cell transcriptomics.Nature communications · 2022Article
- Review
- The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition.Cell reports · 2021Article
- The EHA Research Roadmap: Normal Hematopoiesis.HemaSphere · 2021Article
- Making Blood from the Vessel: Extrinsic and Environmental Cues Guiding the Endothelial-to-Hematopoietic Transition.Life (Basel, Switzerland) · 2021Review
- Lymphoid Tissue inducer (LTi) cell ontogeny and functioning in embryo and adult.Biomedical journal · 2021Review
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The defined location of a stem cell within a niche regulates its fate, behavior, and molecular identity via a complex extrinsic regulation that is far from being fully elucidated. To explore the molecular characteristics and key components of the aortic microenvironment, where the first hematopoietic stem cells are generated during development, we performed genome-wide RNA tomography sequencing on zebrafish, chicken, mouse, and human embryos. The resulting anterior-posterior and dorsal-ventral transcriptional maps provided a powerful resource for exploring genes and regulatory pathways active in the aortic microenvironment. By performing interspecies comparative RNA sequencing analyses and functional assays, we explored the complexity of the aortic microenvironment landscape and the fine-tuning of various factors interacting to control hematopoietic stem cell generation, both in time and space in vivo, including the ligand-receptor couple ADM-RAMP2 and SVEP1. Understanding the regulatory function of the local environment will pave the way for improved stem cell production in vitro and clinical cell therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.