ArticleParasite (Paris, France)2020
A novel nuclear marker and development of an ARMS-PCR assay targeting the metallopeptidase 10 (nas 10) locus to identify the species of the Anisakis simplex (s. l.) complex (Nematoda, Anisakidae).
Article in Parasite (Paris, France), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Determinants of Ascaridoid Nematode Infection and Anisakis-Related Zoonotic Exposure Risk in Eastern Mediterranean Gadiformes Fishes.Transboundary and emerging diseases · 2026Article
- Hybridization and introgression of the mitochondrial genome between the two speciesParasitology · 2025Article
- Anisakidae and Anisakidosis: A Public Health Perspective.Pathogens (Basel, Switzerland) · 2025Review
- Transcriptomic profile of the zoonotic parasiteFrontiers in cellular and infection microbiology · 2025Article
- Anisakis larvae (Nematoda: Anisakidae): retrospective morphological, morphometric, biogeography, and taxonomic status analysis.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2025Article
- Genetic analyses of Anisakis pegreffii (Nematoda: Anisakidae) from the East Asian finless porpoise Neophocaena asiaeorientalis sunameri (Cetacea: Phocoenidae) in Korean waters.Parasitology research · 2024Article
- Re-evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 1: ToRs1-3.EFSA journal. European Food Safety Authority · 2024Article
- Development of a tetra-primer ARMS-PCR for identification of sika and red deer and their hybrids.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- An update and ecological perspective on certain sentinel helminth endoparasites within the Mediterranean Sea.Parasitology · 2023Review
- Population Genetic Structure ofAnimals : an open access journal from MDPI · 2023Article
- Proteomic characterization of extracellular vesicles released by third stage larvae of the zoonotic parasiteFrontiers in cellular and infection microbiology · 2023Article
- Distribution and genetic diversity of Anisakis spp. in cetaceans from the Northeast Atlantic Ocean and the Mediterranean Sea.Scientific reports · 2022Article
- De novo transcriptome assembly and annotation of the third stage larvae of the zoonotic parasite Anisakis pegreffii.BMC research notes · 2022Article
- Hybrid Genotype ofAnimals : an open access journal from MDPI · 2021Article
- Genetic identification of Anisakis spp. (Nematoda: Anisakidae) from cetaceans of the Southwestern Atlantic Ocean: ecological and zoogeographical implications.Parasitology research · 2021Article
- Species composition and infection levels of Anisakis (Nematoda: Anisakidae) in the skipjack tuna Katsuwonus pelamis (Linnaeus) in the Northwest Pacific.Parasitology research · 2021Article
- First report on molecular identification ofJournal of nematology · 2021Article
- Molecular Identification of Zoonotic Parasites of the GenusPathogens (Basel, Switzerland) · 2020Article
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Authors and funding
5 authors.
Funding
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Abstract
The genus Anisakis represents one of the most widespread groups of ascaridoid nematodes in the marine ecosystem. Three closely related taxa are recognized in the Anisakis simplex (s. l.) complex: A. pegreffii, A. simplex (s. s.) and A. berlandi. They are widely distributed in populations of their intermediate/paratenic hosts (fish and squids) and definitive hosts (cetaceans). A novel nuclear gene locus, metallopeptidase 10 (nas 10) (451 bp), was sequenced and validated on a total of 219 specimens of the three species of Anisakis, collected in fish and cetacean hosts from allopatric areas included in their ranges of distribution. The specimens of Anisakis were first identified by allozymes and sequence analysis of the mtDNA cox2 and EF1α-1 nDNA. The novel nuclear marker has shown fixed alternative nucleotide positions in the three species, i.e. diagnostic at 100%, permitting the species determination of a large number of specimens analyzed in the present study. In addition, primers to be used for amplification-refractory mutation system (ARMS) PCR of the same gene locus were designed at these nucleotide positions. Thus, direct genotyping determination, by double ARMS, was developed and validated on 219 specimens belonging to the three species. Complete concordance was observed between the tetra-primer ARMS-PCR assays and direct sequencing results obtained for the nas 10 gene locus. The novel nuclear diagnostic marker will be useful in future studies on a multi-locus genotyping approach and also to study possible hybridization and/or introgression events occurring between the three species in sympatric areas.
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