Evidence map›Paper›PMID 32451643›Full record

ArticleAnalytical and bioanalytical chemistry2020

Monitoring cell endocytosis of liposomes by real-time electrical impedance spectroscopy.

Claudia Caviglia, Francesca Garbarino, Chiara Canali, Fredrik Melander, Roberto Raiteri, Giorgio Ferrari, Marco Sampietro, Arto Heiskanen, Thomas Lars Andresen, Kinga Zór and 1 more

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Claudia CavigliaDepartment of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, Building 423, 2800, Kongens Lyngby, Denmark. claudia.caviglia@gmail.com.
Francesca Garbarino *Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, Building 423, 2800, Kongens Lyngby, Denmark.
Chiara Canali *Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, Building 423, 2800, Kongens Lyngby, Denmark.
Fredrik MelanderDepartment of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, Building 423, 2800, Kongens Lyngby, Denmark.
Roberto RaiteriDepartment of Informatics, Bioengineering, Robotics and System Engineering, University of Genova, Via All'Opera Pia, 11A, 16145, Genoa, Italy.
Giorgio FerrariDepartment of Electronics, Information and Bioengineering, Polytechnic University of Milan, P.za Leonardo da Vinci, 32, 20133, Milan, Italy.
Marco SampietroDepartment of Electronics, Information and Bioengineering, Polytechnic University of Milan, P.za Leonardo da Vinci, 32, 20133, Milan, Italy.
Arto HeiskanenDepartment of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, Building 423, 2800, Kongens Lyngby, Denmark.
Thomas Lars AndresenDepartment of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, Building 423, 2800, Kongens Lyngby, Denmark.
Kinga ZórDepartment of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, Building 423, 2800, Kongens Lyngby, Denmark. kinzo@dtu.dk.
Jenny EmnéusDepartment of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, Building 423, 2800, Kongens Lyngby, Denmark. jemn@dtu.dk.
Technical University of Denmark · DKPolitecnico di Milano · ITUniversity of Genoa · IT

Funding

Danish National Research Foundation IDUN DNRF122EU FP7 project EXCELL NMP4-SL-2008-214706Horizon 2020 Programme, Marie Skłodowska-Curie Innovative Training Network (H2020-MSCA-ITN-2016) Training4CRM 722779Villum Fonden IDUN Grant No. 9301
6 · The paper itself

Abstract

Evaluation and understanding the effect of drug delivery in in vitro systems is fundamental in drug discovery. We present an assay based on real-time electrical impedance spectroscopy (EIS) measurements that can be used to follow the internalisation and cytotoxic effect of a matrix metalloproteinase (MMP)-sensitive liposome formulation loaded with oxaliplatin (OxPt) on colorectal cancer cells. The EIS response identified two different cellular processes: (i) a negative peak in the cell index (CI) within the first 5 h, due to onset of liposome endocytosis, followed by (ii) a subsequent CI increase, due to the reattachment of cells until the onset of cytotoxicity with a decrease in CI. Free OxPt or OxPt-loaded Stealth liposomes did not show this two-stage EIS response; the latter can be due to the fact that Stealth cannot be cleaved by MMPs and thus is not taken up by the cells. Real-time bright-field imaging supported the EIS data, showing variations in cell adherence and cell morphology after exposure to the different liposome formulations. A drastic decrease in cell coverage as well as rounding up of cells during the first 5 h of exposure to OxPt-loaded (MMP)-sensitive liposome formulation is reflected by the first negative EIS response, which indicates the onset of liposome endocytosis. Graphical abstract.

Indexed as

EndocytosisLiposomesAntineoplastic AgentsCell Line, TumorColorectal NeoplasmsDielectric SpectroscopyHumansOxaliplatinAntineoplastic AgentsLiposomesOxaliplatinCell morphologyCytotoxicityElectrical impedance spectroscopyLiposome endocytosisMatrix metalloproteinaseReal-time monitoring

Identifiers

PMID32451643
OpenAlexW3031751938

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.