ArticleNature methods2020
Illumination guidelines for ultrafast pump-probe experiments by serial femtosecond crystallography.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Optimizing photoexcitation conditions for time-resolved X-ray solution scattering experiments.FEBS open bio · 2026Article
- Refinement of amino-acid conformation vs. difference density maps in time-resolved serial femtosecond crystallography data analysis.FEBS open bio · 2026Article
- Quantifying optical scattering losses in pump-probe serial femtosecond crystallography experiments.IUCrJ · 2026Article
- Assessing the problem of excitation light scattering in high-viscosity jets used in serial crystallography sample delivery.Journal of applied crystallography · 2025Article
- Article
- Article
- Observation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography.Communications chemistry · 2025Article
- Review
- Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate.Nature communications · 2024Article
- Article
- A snapshot love story: what serial crystallography has done and will do for us.Acta crystallographica. Section D, Structural biology · 2024Review
- Appraising protein conformational changes by resampling time-resolved serial x-ray crystallography data.Structural dynamics (Melville, N.Y.) · 2024Article
- From femtoseconds to minutes: time-resolved macromolecular crystallography at XFELs and synchrotrons.Acta crystallographica. Section D, Structural biology · 2024Article
- Article
- Micro-structured polymer fixed targets for serial crystallography at synchrotrons and XFELs.IUCrJ · 2023Article
- A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography.Journal of applied crystallography · 2023Article
- Article
- Growing and making nano- and microcrystals.Nature protocols · 2023Review
- Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Biological function investigated by time-resolved structure determination.Structural dynamics (Melville, N.Y.) · 2023Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.