ArticleScientific reports2020
Follicular extracellular vesicles enhance meiotic resumption of domestic cat vitrified oocytes.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
- Ultrastructural Aspects of Oocyte Maturation in Dogs, with Comparative Insights from Cats: Current Evidence and Research Perspectives.Animals : an open access journal from MDPI · 2026Review
- Vitrification of bovine oocytes and embryos: roadmaps to cryopreservation success.Animal reproduction · 2026Article
- Treatment of human oocytes with extracellular vesicles from follicular fluid during rescueHuman reproduction open · 2026Article
- Expanding Horizons: Next-Generation and Interdisciplinary Advances in the Applications of Extracellular Vesicles.Journal of extracellular biology · 2025Review
- Extracellular vesicles in monitoring and modulation of oocyte competence: focus on exosomes.Journal of ovarian research · 2025Review
- Enhancing developmental potential of vitrified in vitro matured bovine oocytes using extracellular vesicles from large follicles.Scientific reports · 2025Article
- Extracellular vesicles from different regions of the female reproductive tract promote spermatozoa motility and support capacitation.Scientific reports · 2025Article
- Follicular-fluid extracellular vesicles support energy metabolism of bovine oocytes, improving blastocyst development and quality†.Biology of reproduction · 2025Article
- Small and Large Extracellular Vesicles From Human Preovulatory Follicular Fluid Display Distinct ncRNA Cargo Profiles and Differential Effects on KGN Granulosa Cells.Journal of extracellular vesicles · 2025Article
- Extracellular vesicles: Roles in oocytes and emerging therapeutic opportunities.Chinese medical journal · 2025Review
- Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis.Journal of extracellular biology · 2025Article
- Extracellular vesicles in female reproduction: from basic research to application.Animal reproduction · 2025Review
- Follicular fluid extracellular vesicles improve bovine oocyte quality via lipid and mitochondrial modulation.Frontiers in veterinary science · 2025Article
- Follicular fluid-derived small extracellular vesicles during individualFrontiers in veterinary science · 2025Article
- Exploring the potential of in vitro extracellular vesicle generation in reproductive biology.Journal of extracellular biology · 2024Review
- Extracellular vesicles and their content in the context of polycystic ovarian syndrome and endometriosis: a review.Journal of ovarian research · 2024Review
- Allogenic Follicular Fosterage Technology: Problems, Progress and Potential.Veterinary sciences · 2024Review
- Rethinking the application of nanoparticles in women's reproductive health and assisted reproduction.Nanomedicine (London, England) · 2024Review
- Extracellular vesicles affecting embryo developmentFrontiers in pharmacology · 2024Review
- Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth.Veterinary sciences · 2022Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Extracellular vesicles (EVs) contain multiple factors that regulate cell and tissue function. However, understanding of their influence on gametes, including communication with the oocyte, remains limited. In the present study, we characterized the proteome of domestic cat (Felis catus) follicular fluid EVs (ffEV). To determine the influence of follicular fluid EVs on gamete cryosurvival and the ability to undergo in vitro maturation, cat oocytes were vitrified using the Cryotop method in the presence or absence of ffEV. Vitrified oocytes were thawed with or without ffEVs, assessed for survival, in vitro cultured for 26 hours and then evaluated for viability and meiotic status. Cat ffEVs had an average size of 129.3 ± 61.7 nm (mean ± SD) and characteristic doughnut shaped circular vesicles in transmission electron microscopy. Proteomic analyses of the ffEVs identified a total of 674 protein groups out of 1,974 proteins, which were classified as being involved in regulation of oxidative phosphorylation, extracellular matrix formation, oocyte meiosis, cholesterol metabolism, glycolysis/gluconeogenesis, and MAPK, PI3K-AKT, HIPPO and calcium signaling pathways. Furthermore, several chaperone proteins associated with the responses to osmotic and thermal stresses were also identified. There were no differences in the oocyte survival among fresh and vitrified oocyte; however, the addition of ffEVs to vitrification and/or thawing media enhanced the ability of frozen-thawed oocytes to resume meiosis. In summary, this study is the first to characterize protein content of cat ffEVs and their potential roles in sustaining meiotic competence of cryopreserved oocytes.
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