Evidence map›Paper›PMID 32450819›Full record

ArticleBMC microbiology2020

Nanopore sequencing reveals genomic map of CTX-M-type extended-spectrum β-lactamases carried by Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway.

Didrik H Grevskott, Francisco Salvà-Serra, Edward R B Moore, Nachiket P Marathe

Open access · goldAbstract read
In one paragraph

Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 22 citations in OpenAlex.

  1. Large DNA fragment ISEc9-mediated transposition during natural transformation allows interspecies dissemination of antimicrobial resistance genes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025
    Article
  2. Article
  3. Article
  4. Detection of drug resistance inFrontiers in veterinary science · 2024
    Article
  5. Review
  6. Strain belonging to an emerging, virulent sublineage of ST131Frontiers in cellular and infection microbiology · 2023
    Article
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 3 countries.

Didrik H GrevskottDepartment of Contaminants and Biohazards, Institute of Marine Research (IMR), Bergen, Norway.
Francisco Salvà-SerraDepartment of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Edward R B MooreDepartment of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Nachiket P MaratheDepartment of Contaminants and Biohazards, Institute of Marine Research (IMR), Bergen, Norway. nachiket.marathe@hi.no.ORCID 0000-0003-2955-3402
Norwegian Institute of Marine Research · NOSahlgrenska University Hospital · SE

Funding

Havforskningsinstituttet 15495Västra Götalandsregionen 51060-6268
6 · The paper itself

Abstract

backgroundEnvironmental surveillance of antibiotic resistance can contribute towards better understanding and management of human and environmental health. This study applied a combination of long-read Oxford Nanopore MinION and short-read Illumina MiSeq-based sequencing to obtain closed complete genome sequences of two CTX-M-producing multidrug-resistant Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway, in order to understand the potential for mobility of the detected antibiotic resistance genes (ARGs).

resultsThe complete genome sequence of strain 631 (E. coli sequence type 38) was assembled into a circular chromosome of 5.19 Mb and five plasmids (between 98 kb and 5 kb). The majority of ARGs cluster in close proximity to each other on the chromosome within two separate multidrug-resistance determining regions (MDRs), each flanked by IS26 transposases. MDR-1 carries bla

conclusionThis study confirms the efficacy of combining Nanopore long-read and Illumina short-read sequencing for determining complete bacterial genome sequences, enabling detection and characterization of clinically important ARGs in the marine environment in Norway, with potential for further dissemination. It also highlights the need for environmental surveillance of antibiotic resistance in low prevalence settings like Norway.

Indexed as

Animalsbeta-LactamasesChromosome MappingConjugation, GeneticDrug Resistance, Multiple, BacterialEnvironmental MonitoringEscherichia coliEscherichia coli InfectionsEscherichia coli ProteinsGenome, BacterialGenome SizeHigh-Throughput Nucleotide SequencingMultigene FamilyMytilus edulisNorwayPhylogenybeta-lactamase CTX-M, E colibeta-LactamasesEscherichia coli ProteinsAntibiotic resistanceESBLEscherichia coliGenome sequenceLong-read sequencingMinIONNanoporeNorway

Identifiers

PMID32450819
PMCPMC7249450
OpenAlexW3032547199

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.