ArticleJournal of visualized experiments : JoVE2020
Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series.
Article in Journal of visualized experiments : JoVE, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Aer is a bidirectional redox sensor mediating negative chemotaxis to antibiotic-induced ROS inmBio · 2026Article
- Texas 2-step: a new model for YcgR::c-di-GMP action at the flagellar motor.Journal of bacteriology · 2025Article
- Texas 2-Step: A new Model for YcgR::c-di-GMP Action at the Flagellar Motor.bioRxiv : the preprint server for biology · 2025Article
- Phenotypic Characterization of Null Mutants of ΔcbpA and ΔcbpB in S. enterica Serovar Typhimurium.Current microbiology · 2025Article
- Effects of microplastic concentration, composition, and size onApplied and environmental microbiology · 2025Article
- Article
- Production and purification of outer membrane vesicles encapsulating green fluorescent protein fromFrontiers in bioengineering and biotechnology · 2024Article
- Swarming Motility Assays in Salmonella.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Efflux-linked accelerated evolution of antibiotic resistance at a population edge.Molecular cell · 2022Article
- Methods to Evaluate Bacterial Motility and Its Role in Bacterial-Host Interactions.Microorganisms · 2022Review
- Surveying a Swarm: Experimental Techniques To Establish and Examine Bacterial Collective Motion.Applied and environmental microbiology · 2022Article
- Article
- The Polar Flagellar Transcriptional Regulatory Network inJournal of bacteriology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Motility is crucial to the survival and success of many bacterial species. Many methodologies exist to exploit motility to understand signaling pathways, to elucidate the function and assembly of flagellar parts, and to examine and understand patterns of movement. Here we demonstrate a combination of three of these methodologies. Motility in soft agar is the oldest, offering a strong selection for isolating gain-of-function suppressor mutations in motility-impaired strains, where motility is restored through a second mutation. The cell-tethering technique, first employed to demonstrate the rotary nature of the flagellar motor, can be used to assess the impact of signaling effectors on the motor speed and its ability to switch rotational direction. The "border-crossing" assay is more recent, where swimming bacteria can be primed to transition into moving collectively as a swarm. In combination, these protocols represent a systematic and powerful approach to identifying components of the motility machinery, and to characterizing their role in different facets of swimming and swarming. They can be easily adapted to study motility in other bacterial species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.