ArticleMolecular systems biology2020
Genetic interaction profiles of regulatory kinases differ between environmental conditions and cellular states.
Article in Molecular systems biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Pooled it
- Experimental Evolution of Yeast Reveals Trade-offs Between Early and Late Stationary Phase.bioRxiv : the preprint server for biology · 2026Article
- Yeast Chronological Lifespan Model as a Tool for Screening Aging Interventions.International journal of molecular sciences · 2026Review
- An Optimized LIVE/DEAD Assay Coupled with Flow Cytometry for Quantifying Post-Stress Survival in Yeast Cells.Journal of visualized experiments : JoVE · 2025Article
- An Optimized LIVE/DEAD Assay Using Flow Cytometry to Quantify Post-Stress and Antifungal-Treatment Survival in Diverse Yeasts.bioRxiv : the preprint server for biology · 2025Article
- Chemical genomic analysis reveals the interplay between iron chelation, zinc homeostasis, and retromer function in the bioactivity of an ethanol adduct of the feijoa fruit-derived ellagitannin vescalagin.G3 (Bethesda, Md.) · 2024Article
- The characteristics of differentiated yeast subpopulations depend on their lifestyle and available nutrients.Scientific reports · 2024Article
- Synthetic viability induces resistance to immune checkpoint inhibitors in cancer cells.British journal of cancer · 2023Article
- scHumanNet: a single-cell network analysis platform for the study of cell-type specificity of disease genes.Nucleic acids research · 2023Article
- Exploring the anti-aging potential of natural products and plant extracts in budding yeastF1000Research · 2023Review
- Quiescence inAnnual review of genetics · 2022Review
- Review
- Phenomics approaches to understand genetic networks and gene function in yeast.Biochemical Society transactions · 2022Article
- Genetic Interaction betweenInternational journal of molecular sciences · 2022Article
- Phosphate Restriction Promotes Longevity via Activation of Autophagy and the Multivesicular Body Pathway.Cells · 2021Article
- Fluctuating Environments Maintain Genetic Diversity through Neutral Fitness Effects and Balancing Selection.Molecular biology and evolution · 2021Article
- High-resolution yeast quiescence profiling in human-like media reveals complex influences of auxotrophy and nutrient availability.GeroScience · 2021Article
- Cellular quiescence in budding yeast.Yeast (Chichester, England) · 2021Review
- Article
- Genetic interaction profiles of regulatory kinases differ between environmental conditions and cellular states.Molecular systems biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cell growth and quiescence in eukaryotic cells is controlled by an evolutionarily conserved network of signaling pathways. Signal transduction networks operate to modulate a wide range of cellular processes and physiological properties when cells exit proliferative growth and initiate a quiescent state. How signaling networks function to respond to diverse signals that result in cell cycle exit and establishment of a quiescent state is poorly understood. Here, we studied the function of signaling pathways in quiescent cells using global genetic interaction mapping in the model eukaryotic cell, Saccharomyces cerevisiae (budding yeast). We performed pooled analysis of genotypes using molecular barcode sequencing (Bar-seq) to test the role of ~4,000 gene deletion mutants and ~12,000 pairwise interactions between all non-essential genes and the protein kinase genes TOR1, RIM15, and PHO85 in three different nutrient-restricted conditions in both proliferative and quiescent cells. We detect up to 10-fold more genetic interactions in quiescent cells than proliferative cells. We find that both individual gene effects and genetic interaction profiles vary depending on the specific pro-quiescence signal. The master regulator of quiescence, RIM15, shows distinct genetic interaction profiles in response to different starvation signals. However, vacuole-related functions show consistent genetic interactions with RIM15 in response to different starvation signals, suggesting that RIM15 integrates diverse signals to maintain protein homeostasis in quiescent cells. Our study expands genome-wide genetic interaction profiling to additional conditions, and phenotypes, and highlights the conditional dependence of epistasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.