ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2020
Phenotypic Analysis of the Mouse Hematopoietic Hierarchy Using Spectral Cytometry: From Stem Cell Subsets to Early Progenitor Compartments.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Glycosylation orchestrates megakaryocytic fate of hematopoietic stem cells via Wnt-MUC13 signaling.Nature communications · 2026Article
- Nitrogen Dioxide Inhalation Disrupts Bone Marrow Hematopoiesis by Inducing Stage-Specific Developmental Blockage in Erythroid and Granulocytic Lineages in Mice.Environment & health (Washington, D.C.) · 2026Article
- Experimental robustness and reproducibility of the murine cecal ligation and puncture sepsis model.Intensive care medicine experimental · 2026Article
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- A unified multimodal single-cell framework reveals a discrete state model of hematopoiesis in mice.Nature immunology · 2025Article
- Interactions of Hematopoietic and Associated Mesenchymal Stem Cell Populations in the Bone Marrow Microenvironment, In Vivo and In Vitro Model.International journal of molecular sciences · 2025Review
- FACS-Based Assessment of Human Hematopoietic Stem and Progenitor Cells.International journal of molecular sciences · 2025Review
- KAT6A chimeras form a self-reinforcing epigenetic module with NURF and MLL/COMPASS to sustain AML.Genome biology · 2025Article
- Gestational diabetes in mice induces hematopoietic memory that affects the long-term health of the offspring.The Journal of clinical investigation · 2024Article
- Slow cycling and durable Flt3+ progenitors contribute to hematopoiesis under native conditions.The Journal of experimental medicine · 2024Article
- Hematopoietic stem cells and extramedullary hematopoiesis in the lungs.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2023Review
- MEK inhibition reduced vascular tumor growth and coagulopathy in a mouse model with hyperactive GNAQ.Nature communications · 2023Article
- Development of Spectral Imaging Cytometry.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Using the Autofluorescence Finder on the Sony ID7000Frontiers in bioengineering and biotechnology · 2022Article
- Hyperspectral multiphoton microscopy forOptica · 2020Article
- FOXO activity adaptation safeguards the hematopoietic stem cell compartment in hyperglycemia.Blood advances · 2020Article
- Full Spectrum Flow Cytometry as a Powerful Technology for Cancer Immunotherapy Research.Frontiers in molecular biosciences · 2020Review
- Angiogenesis in Lymph Nodes Is a Critical Regulator of Immune Response and Lymphoma Growth.Frontiers in immunology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Phenotypic analysis by flow cytometry is one of the most utilized primary tools to study the hematopoietic system. Here, we present a complex panel designed for spectral flow cytometry that allows for the in-depth analysis of the mouse hematopoietic stem and progenitor compartments. The developed panel encompasses the hematopoietic stem cell (HSC) compartment, an array of multipotent progenitors with early marks of lineage specification and a series of progenitors associated with lymphoid, granulo-macrophagic, megakaryocytic and erythroid lineage commitment. It has a built-in redundancy for key markers known to decipher the fine architecture of the HSC compartment by segregating subsets with different functional potential. As a resource, we used this panel to provide a snapshot view of the evolution of these phenotypically defined hematopoietic compartments during the life of the animals. We show that by using a spectral cytometer, this panel is compatible with the analysis of GFP-expressing gene-reporter mice across the hematopoietic system. We leverage this tool to determine how previously described markers such as CD150, CD34, CD105, CD41, ECPR, and CD49b define specific HSC subsets and confirm that high expression of the transcription factor Gfi1 is a hallmark of the most primitive HSC compartment. Altogether, our results provide a convenient protocol to obtain in one analysis a more extensive view of the hematopoietic architecture in mouse models. Our results could also serve as a base for further development of high-end panels leveraging spectral flow cytometry beyond the 15-fluorochrome panel presented in this report. © 2020 International Society for Advancement of Cytometry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.