Evidence map›Paper›PMID 32449090›Full record

ArticleAMB Express2020

Characterization of FsXEG12A from the cellulose-degrading ectosymbiotic fungus Fusarium spp. strain EI cultured by the ambrosia beetle.

Kiyota Sakai, Aya Yamaguchi, Seitaro Tsutsumi, Yuto Kawai, Sho Tsuzuki, Hiromitsu Suzuki, Sadanari Jindou, Yoshihito Suzuki, Hisashi Kajimura, Masashi Kato and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Kiyota SakaiFaculty of Agriculture, Meijo University, Nagoya, Aichi, 468-8502, Japan.
Aya YamaguchiFaculty of Agriculture, Meijo University, Nagoya, Aichi, 468-8502, Japan.
Seitaro TsutsumiFaculty of Agriculture, Meijo University, Nagoya, Aichi, 468-8502, Japan.
Yuto KawaiFaculty of Agriculture, Meijo University, Nagoya, Aichi, 468-8502, Japan.
Sho TsuzukiFaculty of Agriculture, Meijo University, Nagoya, Aichi, 468-8502, Japan.
Hiromitsu SuzukiFaculty of Agriculture, Meijo University, Nagoya, Aichi, 468-8502, Japan.
Sadanari JindouFaculty of Science and Technology, Meijo University, Nagoya, Aichi, 468-8502, Japan.
Yoshihito SuzukiGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 464-8601, Japan.
Hisashi KajimuraGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 464-8601, Japan.
Masashi KatoFaculty of Agriculture, Meijo University, Nagoya, Aichi, 468-8502, Japan.
Motoyuki ShimizuFaculty of Agriculture, Meijo University, Nagoya, Aichi, 468-8502, Japan. moshimi@meijo-u.ac.jp.ORCID http://orcid.org/0000-0002-6907-6367
Meijo University · JPNagoya University · JP

Funding

Grant-in-Aid for Scientific Research 17H03831Grant-in-Aid for Scientific Research 21380055Grant-in-Aid for Scientific Research 25450116Toyoaki Scholarship Foundation H30
6 · The paper itself

Abstract

Despite the threat of Fusarium dieback posed due to ambrosia fungi cultured by ambrosia beetles such as Euwallacea spp., the wood-degradation mechanisms utilized by ambrosia fungi are not fully understood. In this study, we analyzed the 16S rRNA and 18S rRNA genes of the microbial community from the Ficus tree tunnel excavated by Euwallacea interjectus and isolated the cellulose-degrading fungus, Fusarium spp. strain EI, by enrichment culture with carboxymethyl cellulose as the sole carbon source. The cellulolytic enzyme secreted by the fungus was identified and expressed in Pichia pastoris, and its enzymatic properties were characterized. The cellulolytic enzyme, termed FsXEG12A, could hydrolyze carboxymethyl cellulose, microcrystalline cellulose, xyloglucan, lichenan, and glucomannan, indicating that the broad substrate specificity of FsXEG12A could be beneficial for degrading complex wood components such as cellulose, xyloglucan, and galactoglucomannan in angiosperms. Inhibition of FsXEG12A function is, thus, an effective target for Fusarium dieback caused by Euwallacea spp.

Indexed as

Ambrosia beetleEnrichment cultureFusarium diebackFusarium spp.Glycoside hydrolase family 12

Identifiers

PMID32449090
PMCPMC7246284
OpenAlexW3026507809

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.