ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2020
Modifications of fatty acid profile through targeted mutation at BnaFAD2 gene with CRISPR/Cas9-mediated gene editing in Brassica napus.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 124 citations in OpenAlex.
- Genetic Advances inPlants (Basel, Switzerland) · 2026Review
- Unlocking genetic potential: a review of the role of CRISPR/Cas technologies in rapeseed improvement.Stress biology · 2025Review
- Applications of CRISPR/Cas tools in improving stress tolerance inFrontiers in plant science · 2025Review
- Sesame, an Underutilized Oil Seed Crop: Breeding Achievements and Future Challenges.Plants (Basel, Switzerland) · 2024Review
- Targeted mutagenesis of flavonoid biosynthesis pathway genes reveals functional divergence in seed coat colour, oil content and fatty acid composition in Brassica napus L.Plant biotechnology journal · 2024Article
- CRISPR-Cas systems and applications for crop bioengineering.Frontiers in bioengineering and biotechnology · 2024Review
- Transcriptional engineering for value enhancement of oilseed crops: a forward perspective.Frontiers in genome editing · 2024Review
- Modification of Fatty Acid Profile and Oil Contents Using Gene Editing in Oilseed Crops for a Changing Climate.GM crops & food · 2023Review
- KeyInternational journal of molecular sciences · 2023Article
- The current scenario and future perspectives of transgenic oilseed mustard by CRISPR-Cas9.Molecular biology reports · 2023Review
- A novel type of Brassica napus with higher stearic acid in seeds developed through genome editing of BnaSAD2 family.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- CRISPR/Cas9 gene editing technology: a precise and efficient tool for crop quality improvement.Planta · 2023Review
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Review
- Metabolic engineering of plant secondary metabolites: prospects and its technological challenges.Frontiers in plant science · 2023Review
- Improved fatty acid composition of field cress (Frontiers in plant science · 2023Article
- Targeted genome editing in polyploids: lessons fromFrontiers in plant science · 2023Review
- CRISPR-mediated technology for seed oil improvement in rapeseed: Challenges and future perspectives.Frontiers in plant science · 2023Review
- Insights into the NovelGenes · 2022Article
- CRISPR/Cas9-Mediated Gene Editing ofGenes · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
key messageGenomic editing with CRISPR/Cas9 system can simultaneously modify multiple copies of theBnaFAD2 gene to develop novel variations in fatty acids profiles in polyploidy rapeseed. Fatty acid composition affects edible and processing quality of vegetable oil and has been one of the primary targets for genetic modification in oilseed crops including rapeseed (Brassica napus). Fatty acid desaturase 2 gene, FAD2, is a key player that affects three major fatty acids, namely oleic, linoleic and linolenic acid, in oilseed plants. Previously, we showed that there are four copies of BnaFAD2 in allotetraploid rapeseed. In this study, we further established spatiotemporal expression pattern of each copy of BnaFAD2 using published RNA-seq data. Genomic editing technology based on CRISPR/Cas9 system was used to mutate all the copies of BnaFAD2 to create novel allelic variations in oleic acid and other fatty acid levels. A number of mutants at two targeting sites were identified, and the phenotypic variation in the mutants was systematically evaluated. The oleic acid content in the seed of the mutants increased significantly with the highest exceeding 80% compared with wild type of 66.43%, while linoleic and linolenic acid contents decreased accordingly. Mutations on BnaFAD2.A5 caused more dramatic changes of fatty acid profile than the mutations on BnaFAD2.C5 alleles that were identified with gene editing technique for the first time. Moreover, combining different mutated alleles of BnaFAD2 can even broaden the variation more dramatically. It was found that effects of different mutation types at BnaFAD2 alleles on oleic levels varied, indicating a possibility to manipulate fatty acid levels by precise mutation at specific region of a gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.