Evidence map›Paper›PMID 32448280›Full record

ArticleStem cell research & therapy2020

Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro.

Behnaz Ashtari, Azar Shams, Narges Esmaeilzadeh, Sara Tanbakooei, Morteza Koruji, Mojtaba Johari Moghadam, Javad Mohajer Ansari, Adel Johari Moghadam, Ronak Shabani

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Behnaz AshtariShahdad Ronak Commercialization Company, Pasdaran Street, Tehran, Iran.
Azar ShamsCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Narges EsmaeilzadehDepartment of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Sara TanbakooeiSchool of Mechanical Engineering, Iran University of Science & Technology, Tehran, Iran.
Morteza KorujiCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Mojtaba Johari MoghadamDepartment of Cardiology, Aja University of Medical Sciences, Tehran, Iran.
Javad Mohajer AnsariCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Adel Johari MoghadamSchool of Mechanical and Manufacturing Engineering, University of New South Wales, Sydney, New South Wales, 2052, Australia.
Ronak ShabaniCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. shabani.r@iums.ac.ir.
Iran University of Medical Sciences · IRIran University of Science and Technology · IRAja University of Medical Sciences · IRUNSW Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSome children who have survived cancer will be azoospermic in the future. Performing isolation and purification procedures for spermatogonial stem cells (SSC) is very critical. In this regard, performing the process of decontamination of cancerous cells is the initial step. The major objective of the present study is to separate the malignant EL4 cell line in mice and spermatogonial stem cells in vitro.

methodsThe spermatogonial stem cells of sixty neonatal mice were isolated, and the procedure of co-culturing was carried out by EL4 which were classified into 2 major groups: (1) the control group (co-culture in a growth medium) and (2) the group of co-cultured cells which were separated using the microfluidic device. The percentage of cells was assessed using flow cytometry technique and common laboratory technique of immunocytochemistry and finally was confirmed through the laboratory technique of reverse transcription-polymerase chain reaction (RT-PCR).

resultsThe actual percentage of EL4 and SSC after isolation was collected at two outlets: the outputs for the smaller outlet were 0.12% for SSC and 42.14% for EL4, while in the larger outlet, the outputs were 80.38% for SSC and 0.32% for EL4; in the control group, the percentages of cells were 21.44% for SSC and 23.28% for EL4 (based on t test (p ≤ 0.05)).

conclusionsThe present study demonstrates that the use of the microfluidic device is effective in separating cancer cells from spermatogonial stem cells.

Indexed as

Adult Germline Stem CellsSpermatogoniaAnimalsCell Line, TumorCells, CulturedCoculture TechniquesLab-On-A-Chip DevicesMaleMiceCell sortingEL4 cancer cellMicrofluidic devicePurificationSpermatogonial stem cells

Identifiers

PMID32448280
PMCPMC7245899
OpenAlexW3026095006

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.