Evidence map›Paper›PMID 32444918›Full record

ArticleClinical oral investigations2020

Differences between auto-fluorescence and tetracycline-fluorescence in medication-related osteonecrosis of the jaw-a preclinical proof of concept study in the mini-pig.

Oliver Ristow, Dirk Nehrbass, Stephan Zeiter, Daniel Arens, Julius Moratin, Christoph Pautke, Jürgen Hoffmann, Christian Freudlsperger, Sven Otto

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical oral investigations, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Oliver RistowDepartment of Oral and Maxillofacial Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 400, D-69120, Heidelberg, Germany. Oliver.ristow@med.uni-heidelberg.de.ORCID http://orcid.org/0000-0002-3911-262X
Dirk NehrbassAO Research Institute Davos, Clavadelerstr. 8, 7270, Davos, Switzerland.
Stephan ZeiterAO Research Institute Davos, Clavadelerstr. 8, 7270, Davos, Switzerland.
Daniel ArensAO Research Institute Davos, Clavadelerstr. 8, 7270, Davos, Switzerland.
Julius MoratinDepartment of Oral and Maxillofacial Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 400, D-69120, Heidelberg, Germany.
Christoph PautkeDepartment of Oral and Maxillofacial Surgery, Ludwig-Maximilians University of Munich, Lindwurmstr. 2a, D-80336, Munich, Germany.
Jürgen HoffmannDepartment of Oral and Maxillofacial Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 400, D-69120, Heidelberg, Germany.
Christian FreudlspergerDepartment of Oral and Maxillofacial Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 400, D-69120, Heidelberg, Germany.
Sven OttoDepartment of Oral and Maxillofacial Surgery, Ludwig-Maximilians University of Munich, Lindwurmstr. 2a, D-80336, Munich, Germany.
Heidelberg University · DEAO Foundation · CHLudwig-Maximilians-Universität München · DE

Funding

AO Foundation AO Start-up Grant S-16-47R
6 · The paper itself

Abstract

objectivesFluorescence-guided bone surgery is a well-established technique in the treatment of medication-related osteonecrosis of the jaw. No histopathological evidence for bone auto-fluorescence is currently available, and thus, any differences from tetracycline-fluorescence remain unclear. Therefore, the goals of this study were to find out if macroscopic and histological differences occur between the auto- and tetracycline-fluorescence in the delineation of viable and necrotic jawbone in the mini-pig. MATERIALS AND

methodsAccording to the proof of concept, osteonecrosis was provoked in eight Göttingen minipigs. Pigs were divided into two groups (AF group: no fluorochrome label; TF group: tetracycline label). Delineation of necrosis and viable bone was evaluated in vivo and in vitro macro-/microscopically, correlated to fluorescence properties and compared between the two study groups.

resultsNo macroscopic and microscopic clinical differences were seen in fluorescence between the AF and TF groups. Macroscopic and microscopic viable bone fluoresced green, whereas necrotic bone showed no or only pale fluorescence in both groups. The auto-fluorescence was attributable to the arrangements and structure of collagen and the cell-filled bone lacunae.

conclusionNeither in vivo nor in vitro macroscopically differences are apparent between the auto-fluorescence and the tetracycline-fluorescence of bone. The auto-fluorescence is attributable to the arrangements and structure of collagen and the cell-filled bone lacunae. Tetracycline-fluorescence is a mixture of tetracycline (at the bone edges with increased bone formation) and large components of auto-fluorescence. CLINICAL RELEVANCE: Because auto-fluorescence is easy to apply, reproducible, and does not rely on the subjective impression of the surgeon, it promises to be an important standardized alternative to tetracycline-labeled MRONJ therapy.

Indexed as

Bisphosphonate-Associated Osteonecrosis of the JawBone Density Conservation AgentsAnimalsDiphosphonatesFluorescenceProof of Concept StudySwineSwine, MiniatureBone Density Conservation AgentsDiphosphonatesAnimal modelAR-ONJBRONJFluorescence-guided bone surgeryMRONJTherapy

Identifiers

PMID32444918
PMCPMC7666678
OpenAlexW3027728364

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.