Evidence map›Paper›PMID 32444896›Full record

ArticleWorld journal of microbiology & biotechnology2020

Effects of Spo0A on Clostridium acetobutylicum with an emphasis on biofilm formation.

Zhengjiao Yang, Zhenyu Wang, Ming Lei, Jie Zhu, Yuhan Yang, Shishi Wu, Bin Yu, Huanqing Niu, Hanjie Ying, Dong Liu and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in World journal of microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Frontiers in bioengineering and biotechnology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhengjiao YangState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30, Puzhu South Road, Nanjing, 211816, China.
Zhenyu WangState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30, Puzhu South Road, Nanjing, 211816, China.
Ming LeiState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30, Puzhu South Road, Nanjing, 211816, China.
Jie ZhuNanjing Institute of White-Biotech Co. Ltd., Medicine valley Avenue 11, Nanjing, 210032, China.
Yuhan YangState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30, Puzhu South Road, Nanjing, 211816, China.
Shishi WuState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30, Puzhu South Road, Nanjing, 211816, China.
Bin YuState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30, Puzhu South Road, Nanjing, 211816, China.
Huanqing NiuState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30, Puzhu South Road, Nanjing, 211816, China.
Hanjie YingState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30, Puzhu South Road, Nanjing, 211816, China.
Dong LiuState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30, Puzhu South Road, Nanjing, 211816, China. liudong@njtech.edu.cn.ORCID http://orcid.org/0000-0002-3009-7590
Yibing WangSchool of Biotechnology, East China University of Science and Technology, No. 130 Meilong Road, Xuhui District, Shanghai, 200237, China. ybwang@ecust.edu.cn.

Funding

Key Programme 21636003National Key Research and Development Program of China 2018YFA0902200National Key Research and Development Program of China 2018YFB1501705Outstanding Youth Foundation of Jiangsu Province of China SBK2017010373Postgraduate Research & Practice Innovation Program of Jiangsu Province KYCX18_1111the National Nature Science Foundation of China 21706123the Program for Changjiang Scholars and Innovative Research Team in University IRT_14R28
6 · The paper itself

Abstract

Clostridium acetobutylicum is a well-known strain for biofuel production. In previous work, it was found that this strain formed biofilm readily during fermentation processes. Biofilm formation could protect cells and enhance productivities under environmental stresses in our previous work. To explore the molecular mechanism of biofilm formation, Spo0A of C. acetobutylicum was selected to investigate its influences on biofilm formation and other physiological performances. When spo0A gene was disrupted, the spo0A mutant could hardly form biofilm. The aggregation and adhesion abilities of the spo0A mutant as well as its swarming motility were dramatically reduced compared to those of wild type strain. Sporulation was also negatively influenced by spo0A disruption, and solvent production was almost undetectable in the spo0A mutant fermentation. Furthermore, proteomic differences between wild type strain and the spo0A mutant were consistent with physiological performances. This is the first study confirming a genetic clue to C. acetobutylicum biofilm and will be valuable for biofilm optimization through genetic engineering in the future.

Indexed as

Bacterial ProteinsBiofilmsBiofuelsClostridium acetobutylicumFermentationGene Expression ProfilingGene SilencingGenetic EngineeringTranscription FactorsBacterial ProteinsBiofuelsTranscription FactorsBiofilm formationCell motilityClostridium acetobutylicumspo0A

Identifiers

PMID32444896

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.