Evidence map›Paper›PMID 32444459›Full record

ArticleRNA (New York, N.Y.)2020

Influenza A virus utilizes noncanonical cap-snatching to diversify its mRNA/ncRNA.

Lichao Li, Hui Dai, An-Phong Nguyen, Rong Hai, Weifeng Gu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lichao Li *Department of Molecular, Cell, and Systems Biology, University of California, Riverside, California 92521, USA.
Hui Dai *Department of Molecular, Cell, and Systems Biology, University of California, Riverside, California 92521, USA.
An-Phong NguyenDepartment of Molecular, Cell, and Systems Biology, University of California, Riverside, California 92521, USA.
Rong HaiDepartment of Microbiology and Plant Pathology, University of California, Riverside, California 92521, USA.
Weifeng GuDepartment of Molecular, Cell, and Systems Biology, University of California, Riverside, California 92521, USA.

Funding

A Novel RNA Polyphosphatase PIR-1 Plays Important Roles in Silencing Viruses and Regulating Genes in C. elegansR01GM124349 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI GU, WEIFENG · 2018 to 2022
$1.8M
NIGMS NIH HHS R01 GM124349
6 · The paper itself

Abstract

Influenza A virus (IAV) utilizes cap-snatching to obtain host capped small RNAs for priming viral mRNA synthesis, generating capped hybrid mRNAs for translation. Previous studies have been focusing on canonical cap-snatching, which occurs at the very 5' end of viral mRNAs. Here we discovered noncanonical cap-snatching, which generates capped hybrid mRNAs/noncoding RNAs mapped to the region ∼300 nucleotides (nt) upstream of each mRNA 3' end, and to the 5' region, primarily starting at the second nt, of each virion RNAs (vRNA). Like canonical cap-snatching, noncanonical cap-snatching utilizes a base-pairing between the last nt G of host capped RNAs and a nt C of template RNAs to prime RNA synthesis. However, the nt upstream of this template C is usually A/U rather than just U; prime-realignment occurs less frequently. We also demonstrate that IAV can snatch capped IAV RNAs in addition to host RNAs. Noncanonical cap-snatching likely generates novel mRNAs with start AUG encoded in viral or host RNAs. These findings expand our understanding of cap-snatching mechanisms and suggest that IAV may utilize noncanonical cap-snatching to diversify its mRNAs/ncRNAs.

Indexed as

A549 CellsBase PairingCell Line, TumorHumansInfluenza A virusRNA CapsRNA-Dependent RNA PolymeraseRNA, MessengerRNA, UntranslatedRNA, ViralTranscription, GeneticRNA CapsRNA-Dependent RNA PolymeraseRNA, MessengerRNA, UntranslatedRNA, Viralcap-snatchinginfluenza virusnoncoding RNA or ncRNAnovel influenza virus mRNApriming and realignment

Identifiers

PMID32444459
PMCPMC7430677

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.