ArticleEpigenetics & chromatin2020
In vivo chromatin organization on native yeast telomeric regions is independent of a cis-telomere loopback conformation.
Article in Epigenetics & chromatin, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Both genome instability and replicative senescence stem from the shortest telomere in telomerase-negative cells.Nature communications · 2026Article
- A mechanism for telomere-specific telomere length regulation.Nucleic acids research · 2026Article
- A role for nucleosome remodellers during resection of deprotected telomeres in yeast.PloS one · 2026Article
- Subtelomeric elements provide stability to short telomeres in telomerase-negative cells of the budding yeast Naumovozyma castellii.Current genetics · 2025Article
- A mechanism for telomere-specific telomere length regulation.bioRxiv : the preprint server for biology · 2025Article
- TERRA and Telomere Maintenance in the YeastGenes · 2023Review
- Transcription of ncRNAs promotes repair of UV induced DNA lesions in Saccharomyces cerevisiae subtelomeres.PLoS genetics · 2022Article
- Fission yeast Stn1 maintains stability of repetitive DNA at subtelomere and ribosomal DNA regions.Nucleic acids research · 2021Article
- Mechanism of MRX inhibition by Rif2 at telomeres.Nature communications · 2021Article
- Telomere Replication: Solving Multiple End Replication Problems.Frontiers in cell and developmental biology · 2021Review
- Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence.PLoS genetics · 2020Article
- Telomerase biogenesis requires a novel Mex67 function and a cytoplasmic association with the SmeLife · 2020Article
- Twenty years of t-loops: A case study for the importance of collaboration in molecular biology.DNA repair · 2020Review
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2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDNA packaging into chromatin regulates all DNA-related processes and at chromosomal ends could affect both essential functions of telomeres: protection against DNA damage response and telomere replication. Despite this primordial role of chromatin, little is known about chromatin organization, and in particular about nucleosome positioning on unmodified subtelomere-telomere junctions in Saccharomyces cerevisiae.
resultsBy ChEC experiments and indirect end-labeling, we characterized nucleosome positioning as well as specialized protein-DNA associations on most subtelomere-telomere junctions present in budding yeast. The results show that there is a relatively large nucleosome-free region at chromosome ends. Despite the absence of sequence homologies between the two major classes of subtelomere-telomere junctions (i.e.: Y'-telomeres and X-telomeres), all analyzed subtelomere-telomere junctions show a terminal nucleosome-free region just distally from the known Rap1-covered telomeric repeats. Moreover, previous evidence suggested a telomeric chromatin fold-back structure onto subtelomeric areas that supposedly was implicated in chromosome end protection. The in vivo ChEC method used herein in conjunction with several proteins in a natural context revealed no evidence for such structures in bulk chromatin.
conclusionsOur study allows a structural definition of the chromatin found at chromosome ends in budding yeast. This definition, derived with direct in vivo approaches, includes a terminal area that is free of nucleosomes, certain positioned nucleosomes and conserved DNA-bound protein complexes. This organization of subtelomeric and telomeric areas however does not include a telomeric cis-loopback conformation. We propose that the observations on such fold-back structures may report rare and/or transient associations and not stable or constitutive structures.
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