Evidence map›Paper›PMID 32442532›Full record

ArticleStem cell reports2020

A Novel Chemically Differentiated Mouse Embryonic Stem Cell-Based Model to Study Liver Stages of Plasmodium berghei.

Jaishree Tripathi, Charis-Patricia Segeritz, Gareth Griffiths, Wendy Bushell, Ludovic Vallier, William C Skarnes, Maria M Mota, Oliver Billker

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Advancing Key Gaps in the Knowledge ofFrontiers in cellular and infection microbiology · 2022
    Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jaishree TripathiWellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Charis-Patricia SegeritzWellcome Trust and Medical Research Council Stem Cell Institute, Department of Surgery, University of Cambridge, Cambridge, UK.
Gareth GriffithsWellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Wendy BushellWellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Ludovic VallierWellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK; Wellcome Trust and Medical Research Council Stem Cell Institute, Department of Surgery, University of Cambridge, Cambridge, UK.
William C SkarnesThe Jackson Laboratory for Genomic Medicine, Ten Discovery Drive, Farmington, CT 06032, USA.
Maria M MotaUnidade de Malária, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal.
Oliver BillkerWellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK; Molecular Infection Medicine Sweden and Molecular Biology Department, Umeå University, 90187 Umeå, Sweden. Electronic address: oliver.billker@umu.se.

Funding

Medical Research Council G0701448Medical Research Council MC_PC_12009Medical Research Council MC_PC_17230National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/N001540/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/T2T0419
6 · The paper itself

Abstract

Asymptomatic and obligatory liver stage (LS) infection of Plasmodium parasites presents an attractive target for antimalarial vaccine and drug development. Lack of robust cellular models to study LS infection has hindered the discovery and validation of host genes essential for intrahepatic parasite development. Here, we present a chemically differentiated mouse embryonic stem cell (ESC)-based LS model, which supports complete development of Plasmodium berghei exoerythrocytic forms (EEFs) and can be used to define new host-parasite interactions. Using our model, we established that host Pnpla2, coding for adipose triglyceride lipase, is dispensable for P. berghei EEF development. In addition, we also evaluated in-vitro-differentiated human hepatocyte-like cells (iHLCs) to study LS of P. berghei and found it to be a sub-optimal infection model. Overall, our results present a new mouse ESC-based P. berghei LS infection model that can be utilized to study the impact of host genetic variation on parasite development.

Indexed as

Cell DifferentiationHost-Parasite InteractionsAcyltransferasesAnimalsCell LineCells, CulturedHepatocytesHumansLipaseMalariaMiceMouse Embryonic Stem CellsPlasmodium bergheiAcyltransferasesLipasePNPLA2 protein, mouseembryonic stem cellshepatocyte-like cellsmalariamethoxybenzamide differentiationPnpla2

Identifiers

PMID32442532
PMCPMC7355138

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.