ArticlePloS one2020
The cecal and fecal microbiomes and metabolomes of horses before and after metronidazole administration.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- A Comprehensive Review of the Equine Gut Microbiome in Health and Disease.Veterinary sciences · 2026Review
- Evidence for modulation of the fecal microbiota profile by diet in lactating buffalo.Frontiers in veterinary science · 2026Article
- Response of serum biochemical profile, antioxidant enzymes, and gut microbiota to dietary Hong-bailanshen supplementation in horses.Frontiers in microbiology · 2024Article
- Effects of metronidazole on colorectal cancer occurrence and colorectal cancer liver metastases by regulating Fusobacterium nucleatum in mice.Immunity, inflammation and disease · 2023Article
- Changes in the Diversity and Composition of Gut Microbiota of Red-Crowned Cranes (Animals : an open access journal from MDPI · 2022Article
- Caecal microbiota in horses with trigeminal-mediated headshaking.Veterinary medicine and science · 2022Article
- Effects of Intravenous Antimicrobial Drugs on the Equine Fecal Microbiome.Animals : an open access journal from MDPI · 2022Article
- Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses.Frontiers in veterinary science · 2022Article
- Impact of Antibiotic Therapies on Resistance Genes Dynamic and Composition of the Animal Gut Microbiota.Animals : an open access journal from MDPI · 2021Review
- Microbiome in drug resistance to colon cancer.Current opinion in physiology · 2021Article
- Evaluation of changes in microbiota after fecal microbiota transplantation in 6 diarrheic horses.The Canadian veterinary journal = La revue veterinaire canadienne · 2021Article
- Species-Level Gut Microbiota Analysis after Antibiotic-Induced Dysbiosis in Horses.Animals : an open access journal from MDPI · 2021Article
- Changes in the Fecal Microbiota Associated with a Broad-Spectrum Antimicrobial Administration in Hospitalized Neonatal Foals with Probiotics Supplementation.Animals : an open access journal from MDPI · 2021Article
- Alterations in the Fecal Microbiome and Metabolome of Horses with Antimicrobial-Associated Diarrhea Compared to Antibiotic-Treated and Non-Treated Healthy Case Controls.Animals : an open access journal from MDPI · 2021Article
- Multidimensional Approach for Investigating the Effects of an Antibiotic-Probiotic Combination on the Equine Hindgut Ecosystem and Microbial Fibrolysis.Frontiers in microbiology · 2021Article
- The Effect of Ryegrass Silage Feeding on Equine Fecal Microbiota and Blood Metabolite Profile.Frontiers in microbiology · 2021Article
- Inflammation-Associated Microbiota Composition Across Domestic Animals.Frontiers in genetics · 2021Review
- Assessment of clinical and microbiota responses to fecal microbial transplantation in adult horses with diarrhea.PloS one · 2021Article
- Effects of fecal microbiota transplantation on clinical outcomes and fecal microbiota of foals with diarrhea.Journal of veterinary internal medicineArticle
- Effects of orally administered clioquinol on the fecal microbiome of horses.Journal of veterinary internal medicineArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibiotic administration can be a cause of gastrointestinal disease in horses, creating a disruption in the normal population and function of bacteria found in the hindgut. The objective of this study was to describe the changes in the cecal and fecal microbiomes and metabolomes of clinically healthy horses before and after metronidazole administration. Metronidazole (15 mg/kg BID PO) was given to five horses with cecal cannulas. The study was suspended on Day 3 due to adverse gastrointestinal effects. Cecal and fecal samples were obtained before (Days minus52, m28, m14, and 0) and after (Days 7, 14, 28, and 52) metronidazole administration. DNA was extracted from the cecal and fecal samples, and 16S rRNA genes were sequenced. Richness and evenness indices were significantly decreased by metronidazole administration in both cecal and fecal samples, but the overall composition was only significantly changed in fecal samples on Day 3 (ANOSIM, p = 0.008). The most dominant phyla were Bacteroidetes and Firmicutes in all groups examined. In fecal samples, significant changes of the phyla Actinobacteria, Spirochaetes, Lentisphaerae, and Verrucomicrobia occurred on Day 3, which correlated with clinical signs of gastrointestinal disease. The metabolome was characterized by mass spectrometry-based methods and only named metabolites were included in the analysis. Fecal, but not cecal, metabolites were significantly affected by metronidazole. The fecal metabolites affected represent diverse metabolic pathways, such as the metabolism of amino acids, carbohydrates, lipids, nucleic acids and cofactors and vitamins. Metronidazole administration has potential to cause adverse effects in horses, alters the bacterial composition of the horse's cecal and fecal content, and the metabolome of fecal samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.