ArticleAnalytical and bioanalytical chemistry2020
A single-cell identification and capture chip for automatically and rapidly determining hydraulic permeability of cells.
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 8 citations in OpenAlex.
- How does cytoplasmic crowding affect reaction rates?Molecular cell · 2026Review
- Application of antibody-conjugated small intestine submucosa to capture urine-derived stem cells for bladder repair in a rabbit model.Bioactive materials · 2022Article
- Improving Cell Recovery: Freezing and Thawing Optimization of Induced Pluripotent Stem Cells.Cells · 2022Review
- Passive coupling of membrane tension and cell volume during active response of cells to osmosis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The hydraulic permeability of the lipid bilayer membrane of a single cell, a very important parameter in biological and medical fields, has been attracting increasing attention. To date, methods developed to determine this permeability are either operation-complicated or time-consuming. Therefore, we developed a chip for automatically and rapidly determining the permeability of cells that integrates microfluidics and cell impedance analysis. The chip is designed to automatically identify a single cell, capture the cell, and record the volume change in that cell. We confirmed the abilities of single-cell identification and capture with the upper and lower voltage thresholds determined, validated the performance of the differential electrode design for accurate cell volume measurements, deduced the extracellular osmotic pressure change in the presence of a hypertonic solution according to fluorescence intensity, and demonstrated the single-cell volume change recorded by the chip. Then, the accuracy of the permeability determined with the chip was verified using HeLa cells. Finally, the permeability of human-induced pluripotent stem cells (hiPSCs) was determined to be 0.47 ± 0.03 μm/atm/min. Using the chip, the permeability can be determined within 5 min. This study provides insights for the new design of an automatic single-cell identification and capture chip for single cell-related studies. Graphical abstract.
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