Evidence map›Paper›PMID 32440841›Full record

ArticleMolecular and cellular biochemistry2020

Long non-coding RNA H19 promotes osteogenic differentiation of renal interstitial fibroblasts through Wnt-β-catenin pathway.

Zewu Zhu, Yu Cui, Fang Huang, Huimin Zeng, Weiping Xia, Feng Zeng, Cheng He, Jinbo Chen, Zhiyong Chen, Hequn Chen and 1 more

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Molecular and cellular biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 27 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Article
  6. Review
  7. Article
  8. Review
  9. Recent advances on the mechanisms of kidney stone formation (Review).International journal of molecular medicine · 2021
    Review
  10. Article
  11. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Zewu ZhuDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Yu CuiDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Fang HuangDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Huimin ZengDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Weiping XiaDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Feng ZengDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Cheng HeDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Jinbo ChenDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Zhiyong ChenDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Hequn ChenDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Yang LiDepartment of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China. liyangxyyy@163.com.ORCID http://orcid.org/0000-0002-7090-7471
Central South University · CN

Funding

Nature Science Foundation of Hunan province, China 2017JJ2395
6 · The paper itself

Abstract

Randall's plaque (RP) serves as a nidus on which idiopathic calcium oxalate stones form. Renal interstitial mineralization may be the cause underlying RP, and recent studies demonstrated the similarities between the interstitial mineralization and ectopic calcification. The present study aimed to investigate whether human renal interstitial fibroblasts (hRIFs) could form calcification under osteogenic conditions, and whether long non-coding RNA H19 participated in regulating osteogenic differentiation of hRIFs through Wnt-β-catenin pathway. HRIFs were isolated and induced for osteogenic differentiation under osteogenic conditions. Runx2, OCN, alkaline phosphatase (ALP) activity, and the mineralized nodule formation were used to assess the osteogenic phenotype. Molecule expressions were determined by qRT-PCR, immunofluorescence staining, and western blot. The mineralized nodules were assessed by Alizarin red staining. Compared to the normal renal papillary tissue, Runx2, OCN, and H19 were significantly upregulated in RP. After hRIFs were induced with osteogenic medium, osteogenic markers (Runx2, OCN and ALP), β-catenin and H19 were significantly upregulated, and the mineralized nodules are formed. Additionally, overexpression of H19 promoted the osteogenic phenotype of hRIFs and increased the expression of β-catenin, whereas knock-down of H19 or XAV939 (inhibitor of Wnt-β-catenin signaling pathway) significantly repressed the osteogenic phenotype of hRIFs and decreased the β-catenin. Moreover, XAV939 was shown to abolish the osteogenic differentiation of hRIFs promoted by H19. The study demonstrated that ectopic calcification partly participated in the formation of RP, and H19 promoted osteogenic differentiation of hRIFs by activating Wnt-β-catenin pathway, which shed new light on the molecular mechanism of the RP formation.

Indexed as

OsteogenesisWnt Signaling PathwayCell DifferentiationCore Binding Factor Alpha 1 SubunitFibroblastsHumansKidneyMesenchymal Stem CellsPrevalenceRNA, Long NoncodingCore Binding Factor Alpha 1 SubunitH19 long non-coding RNARNA, Long NoncodingRUNX2 protein, humanH19Osteogenic differentiationRandall’s plaqueRenal interstitial fibroblastsβ‐Catenin

Identifiers

PMID32440841
OpenAlexW3028471914

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.